MicroRNA-30d-5p inhibits tumour cell proliferation and motility by directly targeting CCNE2 in non-small cell lung cancer
MicroRNA-30d-5p inhibits tumour cell proliferation and motility by directly targeting CCNE2 in non-small cell lung cancer
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MicroRNA-30d-5p通过直接靶向CCNE2抑制非小细胞肺癌中的肿瘤细胞增殖和运动
DOI：
10.1016/j.canlet.2015.03.041
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发表时间：
2015-07-01
期刊：
影响因子：
9.7
通讯作者：
He, Xianghuo
中科院分区：
文献类型：
--
作者：
Chen, Di;Guo, Weijie;He, Xianghuo
微小RNA（miRNA）是小的、单链的、非编码RNA分子，其在许多类型的人类癌症中失调，尽管它们在驱动非小细胞肺癌（NSCLC）中的精确功能尚不完全清楚。在本研究中，我们发现miR-30 d-5 p在NSCLC组织中经常下调，显著抑制NSCLC细胞的生长、细胞周期分布和运动。此外，我们证明，细胞周期蛋白E2（CCNE 2），这是经常在NSCLC组织中上调，是miR-30 d-5 p的直接目标。CCNE 2表达促进NSCLC细胞的增殖、侵袭和迁移。此外，重新引入CCNE 2表达拮抗了miR-30 d-5 p对NSCLC细胞增殖和运动能力的抑制作用。总之，这些结果表明miR-30 d-5 p/CCNE 2轴可能有助于NSCLC细胞增殖和运动，表明miR-30 d-5 p是治疗NSCLC的潜在治疗靶点。(C)2015爱思唯尔爱尔兰有限公司版权所有。
MicroRNAs (miRNAs) are small, single-stranded, non-coding RNA molecules that are dysregulated in many types of human cancers, although their precise functions in driving non-small cell lung cancer (NSCLC), are incompletely understood. In the present study, we found that miR-30d-5p, often downregulated in NSCLC tissues, significantly inhibited the growth, cell cycle distribution, and motility of NSCLC cells. Furthermore, we demonstrated that cyclin E2 (CCNE2), which was often upregulated in NSCLC tissues, was a direct target of miR-30d-5p. CCNE2 expression promoted the proliferation, invasion, and migration of NSCLC cells. In addition, the re-introduction of CCNE2 expression antagonised the inhibitory effects of miR-30d-5p on the capacity of NSCLC cells for proliferation and motility. Together, these results suggest that the miR-30d-5p/CCNE2 axis may contribute to NSCLC cell proliferation and motility, indicating miR-30d-5p as a potential therapeutic target for the treatment of NSCLC. (C) 2015 Elsevier Ireland Ltd. All rights reserved.