The mitochondrial genome of the phytopathogenic basidiomycete Moniliophthora perniciosa is 109 kb in size and contains a stable integrated plasmid
The mitochondrial genome of the phytopathogenic basidiomycete Moniliophthora perniciosa is 109 kb in size and contains a stable integrated plasmid
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DOI：
10.1016/j.mycres.2008.04.014
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发表时间：
2008-10-01
影响因子：
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通讯作者：
Pereira, Goncalo A. G.
中科院分区：
文献类型：
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作者：
Formighieri, Eduardo F.;Tiburcio, Ricardo A.;Pereira, Goncalo A. G.
我们在这里提出的序列的线粒体基因组的担子菌植物病原性半活体营养真菌Moniliophthora perniciosa，病原体的女巫的扫帚病可可。该DNA是一个环状分子，有109103个碱基对，GC为31.9%，是迄今为止测序的最大的DNA。这种大小主要是由于存在许多非保守的假设ORF。它包含14个编码氧化磷酸化蛋白质的基因，两个rRNA基因，一个编码核糖体蛋白质（rps3）的ORF，以及一组26个识别所有氨基酸密码子的tRNA基因。七个归巢核酸内切酶位于内含子内。除atp8外，所有保守的已知基因都在同一方向上。基于考克斯基因的系统发育分析与公认的真菌分类学一致。这种线粒体基因组的一个不寻常的特征是存在一个区域，该区域包含一组四个相对较小的嵌套反向重复序列，其中包含两个编码具有反转子型结构的聚合酶的基因和三个保守的假设基因，该假设基因被解释为线粒体线性质粒的稳定整合。这种质粒的整合似乎是最近的进化事件，可能对真菌生物学产生影响。该序列的基因库登录号为AY376688。(c)2008年英国真菌学会。由爱思唯尔有限公司出版。保留所有权利。
We present here the sequence of the mitochondrial genome of the basidiomycete phytopathogenic hemibiotrophic fungus Moniliophthora perniciosa, causal agent of the Witches' Broom Disease in Theobroma cacao. The DNA is a circular molecule of 109103 base pairs, with 31.9 % GC, and is the largest sequenced so far. This size is due essentially to the presence of numerous non-conserved hypothetical ORFs. It contains the 14 genes coding for proteins involved in the oxidative phosphorylation, the two rRNA genes, one ORF coding for a ribosomal protein (rps3), and a set of 26 tRNA genes that recognize codons for all amino acids. Seven homing endonucleases are located inside introns. Except atp8, all conserved known genes are in the same orientation. Phylogenetic analysis based on the cox genes agrees with the commonly accepted fungal taxonomy. An uncommon feature of this mitochondrial genome is the presence of a region that contains a set of four, relatively small, nested, inverted repeats enclosing two genes coding for polymerases with an invertron-type structure and three conserved hypothetical genes interpreted as the stable integration of a mitochondrial linear plasmid. The integration of this plasmid seems to be a recent evolutionary event that could have implications in fungal biology. This sequence is available under GenBank accession number AY376688. (c) 2008 The British Mycological Society. Published by Elsevier Ltd. All rights reserved.