Preparation of biologically active 125I-TSH.
Preparation of biologically active 125I-TSH.
复制标题
生物活性125I-TSH的制备。
作者：
I. Goldfine;S. Amir;A. W. Petersen;S. Ingbar
进行本研究以确定是否可以通过允许保留其生物活性的方法制备高比放射性的碘化牛TSH。用化学计量浓度的TSH、Nal和氯胺T，将激素碘化或放射性碘化至每分子11个碘原子的程度。然后通过Sephadex G-25和G-100连续层析从反应混合物中纯化碘化TSH。127 I-TSH在TSH放射免疫测定和McKenzie生物测定中均具有完全效价。I25 I-TSH在生物测定中也具有完全效力，并且至少70%的标记物与纯化的牛甲状腺膜结合。这些研究表明，碘标记的TSH保留生物活性时，采用的方法制备，并以这种方式制备的放射性碘标记的TSH是一个合适的工具，用于研究结合TSH的组织受体。（内分泌学95：1228，1974）
The present study was performed to determine whether iodinated bovine TSH of high specific radioactivity could be prepared by a method that would permit retention of its biological activity. Using stoichiometric concentrations of TSH, Nal, and chloramine T, the hormone was iodinated or radioiodinated to the extent of 1iodine atom per molecule. Iodinated TSH was then purified from the reaction mixture by sequential chromatography on Sephadex G-25 and G-100. 127I-TSH had full potency both in the TSH radioimmunoassay and in the McKenzie bioassay. I25I-TSH also had full potency in the bioassay and at least 70% of the label bound to purified bovine thyroid membranes. These studies indicate that iodinated TSH retains biological activity when prepared by the method employed, and that radioiodinated TSH prepared in this manner is a suitable tool for studying the binding of TSH to its tissue receptors. (Endocrinology 95: 1228, 1974)