Fluorescence in situ hybridization for detecting erbB-2 amplification in breast tumor fine needle aspiration biopsies.
Fluorescence in situ hybridization for detecting erbB-2 amplification in breast tumor fine needle aspiration biopsies.
复制标题
荧光原位杂交用于检测乳腺肿瘤细针抽吸活检中的 erbB-2 扩增。
DOI：
10.1159/000333683
复制
发表时间：
1996
期刊：
影响因子：
1.8
通讯作者：
Waldman,FM
中科院分区：
文献类型：
--
作者：
Sauter,G;Feichter,G;Torhorst,J;Moch,H;Novotna,H;Wagner,U;Dürmüller,U;Waldman,FM
目的探讨细针吸取细胞学(FNA)中erbB-2基因扩增分析的可行性。研究设计：以着丝粒17和erbB-2基因为探针，对58例乳腺癌标本的FNA涂片和游离细胞核进行双标记荧光原位杂交(FISH)检测。结果根据erbB-2/着丝粒17信号比值对肿瘤进行分类。23例为高扩增，4例为低表达，27例为正常。4个肿瘤显示erbB-2缺失，均发生在42岁的患者中。ErbB-2基因扩增与erbB-2免疫组织化学染色阳性密切相关(P<；0001)。分离细胞的FISH分析与FNA活检的FISH分析显示高度符合(P<；0001)。结论
ObjectiveTo evaluate the feasibility of erbB-2 amplification analysis of fine needle aspiration (FNA) biopsies.Study designFNA smears and dissociated nuclei from 58 breast cancer samples were examined by dual-labeling fluorescence in situ hybridization (FISH) with probes for centromere 17 and the erbB-2 gene. The results were compared with the outcome of erbB-2 immunohistochemistry.ResultsTumors were categorized according to the erbB-2/centromere 17 signal ratio. There were 23 tumors with high-level amplification, four cases with a low-level erbB-2 gain and 27 tumors with normal erbB-2 content. Four tumors showed an erbB-2 deletion, all in patients< or= 42 years of age. ErbB-2 amplification was strongly associated with positive erbB-2 immunostaining (P<. 0001). Comparison of FISH analysis on dissociated cells and on FNA biopsies showed high correspondence (P<. 0001).Conclusion