Forensic characteristics and phylogenetic analysis of Hubei Han population in central China using 17 Y-STR loci.
Forensic characteristics and phylogenetic analysis of Hubei Han population in central China using 17 Y-STR loci.
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华中地区湖北汉族人群17个Y-STR基因座的法医学特征及系统发育分析
DOI：
10.1016/j.fsigen.2017.04.013
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Hou Yiping
中科院分区：
文献类型：
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作者：
Wang Zheng;Du Weian;He Guanglin;Liu Jing;Hou Yiping
目前，Y染色体单倍型参考数据库（YHRD，https://yhrd.org，release 53）中最大的国家数据库是中国，其具有大约38000个Y染色体17-标记（Yfiler）单倍型。这些单倍型分布来自中国绝大多数的行政区划，但没有湖北省，这是位于中国中部地区的单倍型数据。本研究选取429名无血缘关系的湖北籍汉族男性个体，对17个Y-STR基因座进行基因分型。共检测到115个等位基因，等位基因频率范围为0.0023 ~ 07506。基因多样性（GD）值在DYS 438的0.3988到DYS 385 a/B的0.9573之间。共获得410个不同的单倍型，总体单倍型多样性（HD）和辨别力（DC）分别为0.9995和0.9557。此外，遗传关系沿着行政（汉族来自不同省份）和民族划分（少数民族）进行了分析，使用分子方差分析（AMOVA）测试和可视化多维标度图（MDS）。包括湖北汉族在内的汉族人群在中国各地表现出很高的遗传同质性，湖北汉族与某些民族之间存在显著的遗传差异，其中以哈萨克族和藏族最为突出。
Currently, the largest national database within the Y chromosome haplotype reference database (YHRD, https://yhrd.org, release 53) is China, which has approximately 38000 Y chromosomal 17-marker (Yfiler) haplotypes. These haplotype profiles derived from the vast majority of Chinese administrative divisions, but no haplotype data was available for Hubei province, which is located in the Central China region. Herein, 429 unrelated male Chinese Han individuals residing in Hubei province were recruited and genotyped with 17 Y-STR loci. 115 alleles were identified with corresponding allele frequencies spanned from 0.0023 to 07506. The gene diversity (GD) values ranged from 0.3988 at DYS438 to 0.9573 at DYS385a/b. A total of 410 distinct haplotypes were obtained with the overall haplotype diversity (HD) and discrimination capacity (DC) was 0.9995 and 0.9557, respectively. Additionally, genetic relationships along administrative (Han Chinese from different provinces) and ethnic divisions (minority ethnic groups) were analyzed using analysis of molecular variance (AMOVA) tests and visualized by multidimensional scaling plots (MDS). The Han ethnicity including the Hubei Han shows a high genetic homogeneity all across China and significant genetic differences existed between the Hubei Han and some ethnic groups, most prominently for the Kazakhs and the Tibetans.