Cardiotrophin-like cytokine induces astrocyte differentiation of fetal neuroepithelial cells via activation of STAT3
Cardiotrophin-like cytokine induces astrocyte differentiation of fetal neuroepithelial cells via activation of STAT3
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类心肌营养素细胞因子通过激活 STAT3 诱导胎儿神经上皮细胞的星形胶质细胞分化
DOI：
10.1006/cyto.2002.1006
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发表时间：
2002-04-07
期刊：
影响因子：
3.8
通讯作者：
Taga, T
中科院分区：
文献类型：
--
作者：
Uemura, A;Takizawa, T;Taga, T
促神经营养素样细胞因子（CLC），又称新的神经营养素-1/B细胞刺激因子-3（NNT-1/BSF-3），是最近发现的白细胞介素（IL）-6细胞因子家族的成员，与gp 130共同作为信号转导受体成分。在这项研究中，我们证明，CLC在胎鼠神经上皮细胞中表达，并有可能诱导其星形胶质细胞分化的协同方式与骨形态发生蛋白（BMP）-2，这也是在胎鼠大脑中表达。CLC刺激导致星形胶质细胞标志物胶质细胞酸性蛋白（GFAP）基因的启动子激活，这明显受到转录因子STAT 3的显性负性形式的表达或启动子中单个STAT 3结合位点的突变的抑制，这些结果表明，CLC通过STAT 3激活在发育中的脑内的星形胶质细胞分化中起作用。(C)2002爱思唯尔科技有限公司版权所有。
Cardiotrophin-like cytokine (CLC), also known as novel neurotrophin-1/B cell stimulating factor-3 (NNT-1/BSF-3), is a recently identified member of the interleukin (IL)-6 family of cytokines that share gp130 as a signal-transducing receptor component. In this study, we demonstrate that CLC is expressed in fetal mouse neuroepithelial cells and has a potential to induce their astrocyte differentiation in a synergistic manner with bone-morphogenetic protein (BMP)-2, which is also expressed in the fetal mouse brain. CLC-stimulation led to promoter activation of the gene for an astrocyte marker, glial fibrillary acidic protein (GFAP), which was clearly inhibited by expression of a dominant negative form of a transcription factor, STAT3, or by introduction of a mutation in a single STAT3-binding site in the promoter, suggesting a critical role of STAT3 in the CLC-induced GFAP transcription.These results suggest that CLC plays a role in astrocyte differentiation via STAT3 activation within the developing brain. (C) 2002 Elsevier Science Ltd. All rights reserved.