Reactive oxygen-induced reactive oxygen formation during human sperm capacitation
Reactive oxygen-induced reactive oxygen formation during human sperm capacitation
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DOI：
10.1016/j.freeradbiomed.2008.11.004
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发表时间：
2009-02-15
影响因子：
7.4
通讯作者：
Lamothe, Genevieve
中科院分区：
文献类型：
--
作者：
de Lamirande, Eve;Lamothe, Genevieve
生理过程通常被细胞产生的超氧阴离子(O-2(中心点))和一氧化氮(无中心点)等活性氧(ROS)激活。我们研究了NO中心点与O-2(中心点-)及其生成物(无中心点合成酶)之间的相互作用。人类精子在获能过程中(获得生育能力所需的转化)中的一氧化氮合酶和一种仍然难以捉摸的氧化酶)。白蛋白、脐血清超滤液、L-精氨酸触发获能和ROS生成(NO中心点和O-2中心点)，超氧化物歧化酶和一氧化氮合酶抑制剂可阻断上述作用。令人惊讶的是，由外源性NO中心点(或O-2(中心点-))引起的获能也被SOD(或NOS抑制剂)阻断。使用的探针在精子上被证明是特异的和无害的。虽然只需要30分钟的O-2(中心点-)，但连续的无中心点的产生对于数小时是必不可少的。获能引起蛋白质酪氨酸硝化的时间依赖性增加，而这一作用可被超氧化物歧化酶和一氧化氮合酶抑制剂阻止，提示O-2(中心点-)和NO。也通过形成ONOO-起作用。没有中心点(或O-2(中心点-))处理的精子启动了剂量依赖的O-2(中心点-)(或无中心点)的产生，首次在细胞中提供了双面ROS诱导的ROS产生的强有力证据。提供的数据显示，在精子获能过程中，NO中心点和O-2(中心点-)及其生成器之间存在密切的相互作用。(C)2008 Elsevier Inc.保留所有权利。
Physiological processes are often activated by reactive oxygen species (ROS), such as the superoxide anion (O-2(center dot-)) and nitric oxide (NO center dot) produced by cells. We studied the interactions between NO center dot and O-2(center dot-), and their generators (NO center dot synthase. NOS, and a still elusive oxidase), in human spermatozoa during capacitation (transformations needed for acquisition of fertility). Albumin, fetal cord serum ultrafiltrate, and L-arginine triggered capacitation and ROS generation (NO center dot and O-2(center dot-)) and superoxide dismutase (SOD) and NOS inhibitors prevented all these effects. Surprisingly, capacitation due to exogenous NO center dot (or O-2(center dot-)) was also blocked by SOD (or NOS inhibitors). Probes used were proven specific and innocuous on spermatozoa. Whereas O-2(center dot-) was needed only for 30 min, the continuous NO center dot generation was essential for hours. Capacitation Caused a time-dependent increase in protein tyrosine nitration that was prevented by SOD and NOS inhibitors, suggesting that O-2(center dot-) and NO. also act via the formation of ONOO-. Spermatozoa treated with NO center dot (or O-2(center dot-)) initiated a dose-dependent O-2(center dot-) (Or NO center dot) production, providing, for the first time in cells, a strong evidence for a two-sided ROS-induced ROS generation. Data presented show a close interaction between NO center dot and O-2(center dot-) and their generators during sperm capacitation. (C) 2008 Elsevier Inc. All rights reserved.