Characterization of the human histone H2A.X gene. Comparison of its promoter with other H2A gene promoters.
Characterization of the human histone H2A.X gene. Comparison of its promoter with other H2A gene promoters.
复制标题
人类组蛋白 H2A.X 基因的表征。
DOI：
10.1016/s0021-9258(19)51067-5
复制
发表时间：
1994
期刊：
影响因子：
--
通讯作者：
William M. Bonner
中科院分区：
文献类型：
--
作者：
Vessela S. Ivanova;C. Hatch;William M. Bonner
非复制链组蛋白H2A的人类基因。X是一种自然产生的嵌合体，它包含一个复制非连锁启动子，但产生一个具有复制连锁组蛋白基因特征的茎环mRNA。符合后一种属性，H2A。X基因缺少内含子。H2A的启动子。X基因定位于转录起始位点上游的120碱基对区域，该区域包含一个TATA和两个CCAAT序列元件。两个CCAAT元素的近端被证明是H2A的重要决定因素。X基因启动子活性。对复制连锁H2A.1a基因和非复制连锁H2A基因的CCAAT元件进行了比较研究。Z基因，H2A的近端CCAAT元件。发现X基因与核因子结合，后者也与CCAAT元件结合，而前者不与CCAAT元件结合。非复制链H2A的特异性。X和H2A。CCAAT结合转录因子的Z基因启动子似乎也位于CCAAT序列两侧约10个碱基对的短同源序列中。
The human gene for the replication-unlinked histone protein H2A.X is a naturally occurring chimera that contains a replication-unlinked promoter yet produces a stemloop mRNA characteristic of replication-linked histone genes. Consistent with the latter attribute, the H2A.X gene was found to lack introns. The promoter of the H2A.X gene was localized to a 120-base pair region upstream of the transcription start site, a region which included a TATA and two CCAAT sequence elements. The proximal of the two CCAAT elements was shown to be an important determinant of H2A.X gene promoter activity. In a comparative study with the CCAAT elements from the replication-linked H2A.1a gene and the replication-unlinked H2A.Z gene, the proximal CCAAT element of the H2A.X gene was found to bind nuclear factors also bound by CCAAT elements in the latter but not in the former. The specificity of the replication-unlinked H2A.X and H2A.Z gene promoters for CCAAT-binding transcription factors appeared to also reside in short homologous sequences about 10 base pairs away on either side of the CCAAT sequence.