Characterization and isolation of highly purified porcine satellite cells.
Characterization and isolation of highly purified porcine satellite cells.
复制标题
高纯度猪的表征和分离
DOI：
10.1038/cddiscovery.2017.3
复制
发表时间：
2017
影响因子：
7
通讯作者：
Hu P
中科院分区：
文献类型：
--
作者：
Ding S;Wang F;Liu Y;Li S;Zhou G;Hu P
猪是一种重要的食物来源，也是人类疾病模型的优良体系。对猪骨骼肌干细胞（卫星细胞）的仔细表征将为猪骨骼肌疾病模型的产生和食品工业中猪肉的高效生产提供线索。Pax 7是一种高度保守的转录因子，在不同物种的卫星细胞中存在。然而，Pax 7的序列尚未在猪中表征。由于缺乏高纯度卫星细胞的分离方法，阻碍了对猪卫星细胞的深入研究。在这里，我们发现了猪卫星细胞的分子标记，并揭示了猪卫星细胞在不同的骨骼肌块是异质性的。我们进一步开发了一种方法，分离高度纯化的卫星细胞直接从猪肌肉使用荧光激活细胞分选。我们接下来表征了分离的卫星细胞在体外的增殖和分化能力;并且发现卫星细胞在体外的长期培养导致干性丧失。
Pig is an important food source and an excellent system to model human diseases. Careful characterization of the swine skeletal muscle stem cells (satellite cells) will shed lights on generation of swine skeletal muscle disease model and efficient production of porcine meat for the food industry. Paired box protein 7 (Pax7) is a highly conserved transcription factor shared by satellite cells from various species. However, the sequence of Pax7 has not been characterized in pig. The lack of method to isolate highly purified satellite cells hinders the thorough characterization of the swine satellite cells. Here we found molecular markers for swine satellite cells and revealed that the porcine satellite cells were heterogeneous in various pieces of skeletal muscle. We further developed a method to isolate highly purified satellite cells directly from porcine muscles using fluorescence-activated cell sorting. We next characterized the proliferation and differentiation abilities of isolated satellite cells in vitro; and found that long-term culturing of satellite cells in vitro led to stemness loss.