Effect of nutrient deprivation on the susceptibility of Galleria mellonella larvae to infection
Effect of nutrient deprivation on the susceptibility of Galleria mellonella larvae to infection
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DOI：
10.4161/viru.21972
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发表时间：
2012-10-01
期刊：
影响因子：
5.2
通讯作者：
Kavanagh, Kevin
中科院分区：
文献类型：
--
作者：
Banville, Nessa;Browne, Niall;Kavanagh, Kevin
大蜡螟幼虫被广泛用于研究微生物病原体的毒力和评估抗菌剂的效力。这项工作研究了营养缺乏对幼虫抵御感染能力的影响，以建立标准化的条件，用于体内试验的幼虫治疗。被剥夺食物七天的幼虫表现出对酵母菌白色念珠菌感染的易感性增加。与对照相比，这些幼虫显示出较低的血细胞密度，但来自饥饿和对照幼虫的血细胞表现出相同的杀死酵母细胞的能力。来自饥饿幼虫的血淋巴显示出一系列抗微生物肽（例如，脂质运载蛋白）和免疫蛋白（例如，载脂蛋白和芳基蛋白）。D. G.大蜡螟幼虫的食物会导致细胞和免疫反应的减少，并增加对感染的易感性。利用这些幼虫的研究人员应确保为幼虫提供足够的食物，以便在不同实验室的结果之间进行有效的比较。
Larvae of Galleria mellonella are widely used to study the virulence of microbial pathogens and for assessing the potency of antimicrobial agents. This work examined the effect of nutritional deprivation on the ability of larvae to withstand infection in order to establish standardized conditions for the treatment of larvae for in vivo testing. Larvae deprived of food for seven days demonstrated an increased susceptibility to infection by the yeast Candida albicans. These larvae displayed a lower density of hemocytes compared with controls but hemocytes from starved and control larvae demonstrated the same ability to kill yeast cells. Hemolymph from starved larvae demonstrated reduced expression of a range of antimicrobial peptides (e.g., lipocalin) and immune proteins (e.g., apolipophorin and arylphorin). Deprivation of G. mellonella larvae of food leads to a reduction in the cellular and immune responses and an increased susceptibility to infection. Researchers utilizing these larvae should ensure adequate food is provided to larvae in order to allow valid comparisons to be made between results from different laboratories.