GENETIC-VARIANTS WITHIN THE GENUS ECHINOCOCCUS IDENTIFIED BY MITOCHONDRIAL-DNA SEQUENCING
GENETIC-VARIANTS WITHIN THE GENUS ECHINOCOCCUS IDENTIFIED BY MITOCHONDRIAL-DNA SEQUENCING
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DOI：
10.1016/0166-6851(92)90109-w
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发表时间：
1992-09-01
影响因子：
1.5
通讯作者：
MCMANUS, DP
中科院分区：
文献类型：
--
作者：
BOWLES, J;BLAIR, D;MCMANUS, DP
棘球绦虫属内的物种和菌株变异的模式是复杂和有争议的。为了客观地鉴定不同的种和株，测定了56株棘球绦虫分离株的线粒体细胞色素c氧化酶亚基I（CO1）基因的一个区域的序列。检测到11种不同的基因型，包括细粒棘球绦虫内的7种，并将这些基因型用于对分离株进行分类。使用这种方法，4种普遍接受的棘球绦虫属物种明显可区分。此外，E.根据各种不同的分化标准，颗粒肌群被广泛支持。在多房棘球绦虫内检测到非常小的变异。在DNA序列水平上发现了显著的菌株内均一性。这个快速进化的线粒体基因组区域可用作物种和菌株身份的标记，并作为棘球绦虫属内进化分歧的初步指示。
The pattern of species and strain variation within the genus Echinococcus is complex and controversial. In an attempt to characterise objectively the various species and strains, the sequence of a region of the mitochondrial cytochrome c oxidase subunit I (CO1) gene was determined for 56 Echinococcus isolates. Eleven different genotypes were detected, including 7 within Echinococcus granulosus, and these were used to categorise the isolates. The 4 generally accepted Echinococcus species were dearly distinguishable using this approach. In addition, the consensus view of the strain pattern within E. granulosus, based on a variety of criteria of differentiation, was broadly upheld. Very little variation was detected within Echinococcus multilocularis. Remarkable intra-strain homogeneity was found at the DNA sequence level. This region of the rapidly evolving mitochondrial genome is useful as a marker of species and strain identity and as a preliminary indication of evolutionary divergence within the genus Echinococcus.