Estrogen regulation of yolk and non-yolk protein synthesis in the avian liver. An immunocytochemical study.
Estrogen regulation of yolk and non-yolk protein synthesis in the avian liver. An immunocytochemical study.
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雌激素对禽肝脏中卵黄和非卵黄蛋白合成的调节。
DOI：
10.1111/j.1432-0436.1981.tb01110.x
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发表时间：
1981
期刊：
影响因子：
--
通讯作者：
Chan,L
中科院分区：
文献类型：
--
作者：
Lin,CT;Chan,L
用免疫细胞化学技术研究了急、慢性雌激素处理对卵黄蛋白(卵黄蛋白原)和载脂蛋白-II(ApoVLDL-II)以及两种非蛋黄蛋白(卵清蛋白和载脂蛋白A-I)的影响。3组公鸡在处死前24 h不处理，或一次性注射己烯雌酚(DES，2.5 mg)(急性刺激)，或在处死前每天注射DES 2.5 mg(慢性刺激)。在4周龄时处死动物，用间接免疫过氧化物酶方法检查其肝脏中四种不同蛋白质的分布。未经治疗的公鸡肝脏中未检测到卵黄蛋白原。单次注射DES可使约10%-15%的肝细胞出现该蛋白。慢性DES刺激使阳性细胞数增加到20%左右。相反，在未经治疗的动物中，apoVLDL-II存在于1%-2%的肝细胞中。在单剂量DES后，在细胞中检测到它的比例增加(20%-25%)。在慢性雌激素治疗后，阳性细胞数量有非常显著的增加(>；90%)。在未经处理的公鸡肝脏中未检测到卵清蛋白，而在极低比例的细胞中检测到载脂蛋白A-I(0.005%-0.01%)。无论是卵白蛋白还是apoA-I的分布似乎都不受单剂量DES的影响。然而，慢性DES处理导致卵清蛋白细胞的出现(∼0.02%)和含有apoA-I的细胞数量显著增加(10%-15%)。
The effects of acute and chronic estrogen treatment on two egg yolk proteins, vitellogenin and apoVLDL‐II, and two non‐yolk proteins, ovalbumin and apoA‐I, were studied by immunocytochemical techniques. Three groups of cockerels received either no treatment, or a single injection of diethylstilbestrol (DES, 2.5 mg) (acute stimulation) 24 h before killing, or 14 daily injections of 2.5 mg DES (chronic stimulation) before killing. The animals were killed at 4 weeks of age and their livers examined with respect to the distribution of the four different proteins by the indirect immunoperoxidase method. Vitellogenin was undetectable in the untreated cockerel liver. A single injection of DES resulted in the appearance of the protein in approximately 10%–15% of the hepatocytes. Chronic DES stimulation increased the number of positive cells to about 20%. In contrast, apoVLDL‐II was present in 1%–2% of the hepatocytes in untreated animals. It was detected in an increased proportion (20%–25%) of cells after a single dose of DES. After chronic estrogen treatment, there was a very marked increase in the number of positive cells (> 90%). Ovalbumin was undetectable in untreated cockerel liver, while apoA‐I was detected in an extremely low proportion of cells (0.005%–0.01%). Neither ovalbumin nor apoA‐I distribution seemed to be affected by a single dose of DES. However, chronic DES treatment resulted in the appearance of ovalbumin‐containing cells (∼ 0.02%) and a marked increase in the number of cells containing apoA‐I (10%–15%).