Discovery of a second form of tripartite complex containing gH-gL of human herpesvirus 6 and observations on CD46
Discovery of a second form of tripartite complex containing gH-gL of human herpesvirus 6 and observations on CD46
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DOI：
10.1128/jvi.78.9.4609-4616.2004
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发表时间：
2004-05-01
影响因子：
5.4
通讯作者：
Yamanishi, K
中科院分区：
文献类型：
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作者：
Mori, Y;Akkapaiboon, P;Yamanishi, K
人疱疹病毒6（HRV-6）糖蛋白H（gH）-糖蛋白L（gL）复合物与糖蛋白Q（gQ）缔合（Y. Mori，P. Akkapaiboon，X. Yang和K. Yamanishi，J. Virol. 77：2452- 2458，2003），并且gH-gL-gQ复合物与人CD 46相互作用（Y. Mori，X. Yang，P. Akkapaiboon，T. Okuno和K. Yamanishi，J. Virol. 77：4992-4999，2003）。在这里，我们表明，HHV-6 U47基因，这是一个位置同源的人巨细胞病毒糖蛋白O（gO）基因，编码的第三个组成部分的HHV-6 gH-gL的包膜复合物。抗HHV-6 gO氨基末端的单克隆抗体（MAb）在免疫印迹中与HHV-6感染细胞裂解物中120至130 kDa和74至80 kDa迁移的蛋白质种类反应，并与纯化病毒体中74至80 kDa的蛋白质种类反应。80-kDa形式的gO与抗gH单克隆抗体共免疫沉淀，但抗gQ MAII，其共免疫沉淀gH，不共沉淀gO。此外，gH-gL-gO复合物不与人CD 46结合，表明该复合物不是CD 46的配体。这些结果表明，病毒包膜至少含有gH-gL-gQ和gH-gL-gO两种三元复合物，gH-gL-gO复合物在病毒感染过程中可能起着与gH-gL-gQ不同的作用。这是首次报道疱疹病毒科成员的病毒包膜上存在两种gH-gL复合物。
The human herpesvirus 6 (HRV-6) glycoprotein H (gH)-glycoprotein L (gL) complex associates with glycoprotein Q (gQ) (Y. Mori, P. Akkapaiboon, X. Yang, and K. Yamanishi, J. Virol. 77:2452-2458,2003), and the gH-gL-gQ complex interacts with human CD46 (Y. Mori, X. Yang, P. Akkapaiboon, T. Okuno, and K. Yamanishi, J. Virol. 77:4992-4999, 2003). Here, we show that the HHV-6 U47 gene, which is a positional homolog of the human cytomegallovirus glycoprotein O (gO) gene, encodes a third component of the HHV-6 gH-gL-containing envelope complex. A monoclonal antibody (MAb) against the amino terminus of HHV-6 gO reacted in immunoblots with protein species migrating at 120 to 130 kDa and 74 to 80 kDa in lysates of HHV-6-infected cells and with a 74- to 80-kDa protein species in purified virions. The 80-kDa form of gO was coimmunoprecipitated with an anti-gH MAb, but an anti-gQ MAII, which coimmunoprecipitated gH, did not coprecipitate gO. Furthermore, the gH-gL-gO complex did not bind to human CD46, indicating that the complex was not a ligand for CD46. These findings suggested that the viral envelope contains at least two kinds of tripartite complexes, gH-gL-gQ and gH-gL-gO, and that the gH-gL-gO complex may play a role different from that of gH-gL-gQ during viral infection. This is the first report of two kinds of gH-gL complexes on the viral envelope in a member of the herpesvirus family.