Association of ALOX5, LTA4H and LTC4S gene polymorphisms with ischemic stroke risk in a cohort of Chinese in east China
Association of ALOX5, LTA4H and LTC4S gene polymorphisms with ischemic stroke risk in a cohort of Chinese in east China
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DOI：
10.5847/wjem.j.1920-8642.2013.01.006
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发表时间：
2013-03-01
影响因子：
2.1
通讯作者：
Xiao, Hang
中科院分区：
文献类型：
--
作者：
Wang, Gan-nan;Zhang, Jin-song;Xiao, Hang
背景：5-脂氧合酶激活蛋白和白三烯 A4 水解酶基因的遗传变异导致缺血性中风风险增加，表明白三烯家族是缺血性中风的潜在介质。本研究旨在探讨华东地区人群中ALOX5、LTA4H和LTC4S基因多态性与缺血性脑卒中风险的关系。方法：本病例对照研究包括690名缺血性脑卒中患者和690名对照者。通过聚合酶链反应和限制性片段长度多态性 (PCR-RFLP) 分析对 ALOX5 rs2029253 A/G、LTA4H rs6538697 TIC 和 LTC4S rs730012 A/C 的多态性进行基因分型。采用多因素logistic回归模型排除常规危险因素对缺血性脑卒中的影响。结果：rs730012位点C等位基因携带者更易患缺血性脑卒中（OR：1.37；95%Cl：1.08~1.73；P=0.009）。 rs2029253 GG基因型显示出对缺血性中风的风险降低作用（OR：0.72；95%Cl：0.55-0.93；P=0.013），而rs6538697 CC基因型则具有增加缺血性中风的风险（OR：1.77；95%Cl：1.09-2.89；P=0.013）。 P=0.022）。调整混杂因素后，rs730012变异与缺血性脑卒中风险无关（P>0.05）。 结论：本研究表明，白三烯通路基因多态性可能对华东地区人群的缺血性脑卒中易感性产生独立的遗传效应。
BACKGROUND: Genetic variations of the 5-lipoxygenase activating protein and leukotriene A4 hydrolase genes that confer an increased risk of ischemic stroke have implicated the family of leukotrienes as potential mediators of ischemic stroke. This study aimed to explore the association of ALOX5, LTA4H and LTC4S gene polymorphisms with ischemic stroke risk in a cohort of Chinese in east China.METHODS: This case-control study consisted of 690 patients with ischemic stroke and 690 controls. Polymorphisms of ALOX5 rs2029253 A/G, LTA4H rs6538697 TIC, and LTC4S rs730012 A/C were genotyped by the polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) analysis. The multivariate logistic regression model was used to exclude the effects of conventional risk factors on ischemic stroke.RESULTS: Carriers of C allele in rs730012 were more susceptible to ischemic stroke (OR: 1.37; 95%Cl: 1.08-1.73; P=0.009). The rs2029253 GG genotype showed a risk -reducing effect on ischemic stroke (OR: 0.72; 95%Cl: 0.55-0.93; P=0.013) while the rs6538697 CC genotype had an increased risk of ischemic stroke (OR: 1.77; 95%Cl: 1.09-2.89; P=0.022). The rs730012 variant was not associated with ischemic stroke risk after adjusting confounding factors (P>0.05).CONCLUSION: The present study suggested that gene polymorphisms in the leukotrienes pathway may exert influences, with independent genetic effects, on ischemic stroke susceptibility in a cohort of Chinese in east China.