Expression of chromosome 21 specific sequences in normal and Down's syndrome tissues.
Expression of chromosome 21 specific sequences in normal and Down's syndrome tissues.
复制标题
21 号染色体特定序列在正常和唐氏综合症组织中的表达。
DOI：
10.1093/nar/16.7.2885
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发表时间：
1988
影响因子：
14.9
通讯作者：
N. Nevin
中科院分区：
文献类型：
--
作者：
L. Stefani;J. Galt;A. Palmer;N. Affara;M. Ferguson;N. Nevin
使用从年龄和性别匹配的正常和唐氏综合症胎儿肝脏和脑组织中分离的RNA，制备Northern印迹并用4条21号染色体特定序列进行探测。结果表明，当唐氏综合症组织与正常组织进行比较时，没有出现一致的表达模式，但结果与预期的3/2表达比率有很大不同。两个序列21.3和26C显示在21三体肝脏样本中的表达仅存在微小差异，但当比较正常和唐氏综合症脑样本时其表达模式发生显着变化。其他序列 JG77 和 JG90 在唐氏综合症的大脑中显示出高 5 倍的表达程度，但当比较肝脏样本时，其中一个序列在正常和唐氏综合症样本中显示出相同的表达水平，而另一个序列在唐氏综合症样本中显示出表达水平降低。
Using RNA isolated from age and sex matched normal and Down's Syndrome foetal liver and brain tissues, Northern blots were prepared and probed with 4 chromosome 21 specific sequences. The results show that no consistent pattern of expression emerges when Down's Syndrome tissue is compared with normal tissue but the results are very different from the 3/2 ratio of expression which may be expected. Two sequences 21.3 and 26C show only minor differences in expression in trisomy 21 liver samples but significant changes in their expression pattern when normal and Down's Syndrome brain samples are compared. The other sequences, JG77 and JG90 show a 5 fold higher degree of expression in Down's brain but when liver samples are compared one of these sequences shows equal levels of expression in normal and Down's Syndrome samples and the other shows a decrease in expression level in Down's Syndrome samples.