Role of Pseudomonas aeruginosa quorum-sensing systems in a mouse model of chronic respiratory infection
Role of Pseudomonas aeruginosa quorum-sensing systems in a mouse model of chronic respiratory infection
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DOI：
10.1099/jmm.0.46004-0
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发表时间：
2005-06-01
影响因子：
3
通讯作者：
Kohno, S
中科院分区：
文献类型：
--
作者：
Imamura, Y;Yanagihara, K;Kohno, S
研究了群体感应系统在慢性铜绿假单胞菌感染小鼠模型中的作用。通过在支气管中放置预先包被有菌株PAO 1（野生型）或群体感应突变体，即PAO-JP 1 Δ lasl））、PDO 100（Δ rhl）或PAO-JP 2（Δ lasl Δ rhl）的管，建立慢性铜绿假单胞菌呼吸道感染模型。在感染后第14天，群体感应突变体组中的活菌数量低于野生型组。组织学检查显示，与野生型组相比，突变体组感染的肺部炎症变化较轻。在群体感应系统突变组的支气管肺泡灌洗液中，中性粒细胞的比例低于野生型组。这些发现表明群体感应系统在慢性铜绿假单胞菌呼吸道感染中起重要作用。
The role of quorum-sensing systems in a mouse model of chronic Pseudomonas aeruginosa infection was studied. A chronic P. aeruginosa respiratory infection model was established by placement of a tube pre-coated with strain PAO1 (wild-type) or a quorum-sensing mutant, namely PAO-JP1 Delta lasl)), PDO100 (Delta rhl/) or PAO-JP2 (Delta lasll Delta rhl, in the bronchus. At day 14 after infection, the numbers of viable bacteria in the quorum-sensing-mutant groups were lower than in the wild-type group. Histopathological examination showed milder inflammatory changes in the lungs infected with the mutant groups compared with the wild-type group. In the bronchoalveolar lavage fluid from the quorum-sensing-system-mutant groups the proportion of neutrophils was lower than in wild-type group. These findings indicate that the quorum-sensing system plays an important role in chronic P. aeruginosa respiratory infection.