DIFFERENTIAL EXPRESSION AND FUNCTION OF CADHERIN-LIKE PROTEINS IN THE SEA-URCHIN EMBRYO
DIFFERENTIAL EXPRESSION AND FUNCTION OF CADHERIN-LIKE PROTEINS IN THE SEA-URCHIN EMBRYO
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DOI：
10.1016/0925-4773(93)90054-2
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发表时间：
1993-04-01
影响因子：
2.6
通讯作者：
VITTORELLI, ML
中科院分区：
文献类型：
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作者：
GHERSI, G;SALAMONE, M;VITTORELLI, ML
钙粘蛋白是Ca+2依赖性的细胞表面蛋白，参与细胞粘附特性的规范。它们被认为在形态发生和图案形成中起着关键作用。在本文中，我们表明，在海胆胚胎中至少有两种不同的钙粘蛋白的相对分子质量为140和125 kDa。140 kDa的钙粘蛋白已经存在于受精卵中，是E-钙粘蛋白的海胆等价物。125 kDa的钙粘蛋白，可以检测到使用广谱抗钙粘蛋白抗体，只出现在发展的后期阶段。在后来的胚胎中，这两种分子分布不同：E-钙粘蛋白主要存在于原肠胚的内陷内胚层中，而125 kDa蛋白存在于大多数上皮细胞的细胞表面上。与观察到的表达和分布差异一致，针对这两种钙粘蛋白的抗体不同地干扰海胆发育。例如，当这些抗体被添加到早期原肠胚中时，只有针对125 kDa组分的抗体可以诱导外胚层的完全解聚，而抗E-钙粘蛋白抗体诱导内胚层的异常发育，同时胚胎保持其基本完整性。这些结果进行了讨论，鉴于需要多个粘附受体在图案形成和胚胎发生。
Cadherins are Ca+2-dependent cell surface proteins involved in the specification of the adhesive properties of cells. They are supposed to play a critical role in morphogenesis and pattern formation. In this paper we show that in the sea urchin embryo there are at least two different cadherins of relative molecular masses 140 and 125 kDa. The 140 kDa cadherin is already present in the fertilized egg and is the sea urchin equivalent of E-cadherin. The 125 kDa cadherin, which can be detected using a broad-spectrum anti-cadherin antibody, appears only at later stages of development. In later embryos these two molecules are distributed differently: E-cadherin is present predominantly in the invaginating endoderm of the gastrula while the 125 kDa protein is present on the cell surface of most epithelia. Consistently with the observed differences in expression and in distribution, antibodies directed against these two cadherins differently perturb sea urchin development. For example, when these antibodies are added to early gastrulas only the antibodies against the 125 kDa component can induce a complete disaggregation of the ectoderm, while anti E-cadherin antibodies induce an abnormal development of the endoderm while the embryo maintains its basic integrity. These results are discussed in view of the need for multiple adhesion receptors during pattern formation and embryogenesis.