Quantitative and Site-Specific Chemoproteomic Profiling of Targets of Acrolein
Quantitative and Site-Specific Chemoproteomic Profiling of Targets of Acrolein
复制标题
丙烯醛靶标的定量和位点特异性化学蛋白质组学分析
DOI：
10.1021/acs.chemrestox.8b00343
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发表时间：
2019-03-01
影响因子：
4.1
通讯作者：
Wang, Chu
中科院分区：
文献类型：
--
作者：
Chen, Ying;Liu, Yuan;Wang, Chu
丙烯醛存在于香烟、汽车尾气等常见污染物中，与多种病理过程有关。它也是一种内源性脂质衍生电泳体(LDE)，当细胞处于氧化应激状态时，由脂质过氧化产生。在化学上，丙烯醛能够共价修饰蛋白质中的亲核残基，从而影响蛋白质的结构和功能，而在蛋白质组中识别丙烯醛修饰的靶标是理解其生物学作用的关键。在这里，我们报告了一种定量的化学蛋白质组学方法，使用以醛为导向的苯胺为基础的探针来全局描述丙烯醛修饰。总体而言，我们确定了丙烯醛靶向的2300个蛋白质和500个半胱氨酸位点。我们的数据为理解丙烯醛介导的毒性和扩大我们对氧化应激介导的损伤和信号转导的知识提供了有价值的信息。
Acrolein exists in common pollutants, such as cigarette smoke and car exhaust, which has been implicated with many pathological processes. It is also one type of endogenous lipid-derived electrophile (LDE) generated from lipid peroxidation when cells are under oxidative stress. Chemically, acrolein is able to covalently modify nucleophilic residues in proteins so as to influence their structures and functions, and identification of targets of acrolein modification in proteomes is critical for understanding its biological roles. Here, we report a quantitative chemoproteomic method to globally profile acrolein modifications using an aldehyde-directed aniline-based probe. Collectively, we identified >2300 proteins and >500 cysteine sites that are targeted by acrolein. Our data provide valuable information for understanding acrolein-mediated toxicity and expanding our knowledge of oxidative stress-mediated damage and signaling.