Metyrapone interaction with Pseudomonas putida cytochrome P-450
Metyrapone interaction with Pseudomonas putida cytochrome P-450
复制标题
美替拉酮与恶臭假单胞菌细胞色素 P-450 的相互作用
DOI：
10.1016/s0003-9861(71)80013-9
复制
发表时间：
1971
影响因子：
3.9
通讯作者：
A. Hildebrandt
中科院分区：
文献类型：
--
作者：
J. Peterson;V. Ullrich;A. Hildebrandt
描述了腐殖假单胞菌P-450与甲基甲拉酮（2-甲基-1,2-二-3-吡啶-1-丙酮）的结合。铁形式的甲替rapon -细胞色素P-450配合物的绝对吸收光谱在421和536 nm处有最大吸收。在亚铁形式下，该配合物在442、539和566 nm处具有最大吸收。氧化细胞色素P-450与甲基曲酮结合的平衡常数为2.3×108m−1，与底物樟脑的结合具有竞争性。Metyrapone抑制了偶联单加氧酶系统对氧的摄取，可能是由于与樟脑竞争氧化细胞色素P-450上的樟脑结合位点。结果表明，樟脑结合位点靠近细胞色素P-450上的氧活化位点。
The binding of metyrapone (2-methyl-1,2-di-3-pyridyl-1-propanone) byPseudomonas putidacytochrome P-450 is described. The absolute absorption spectrum of the metyrapone-cytochrome P-450 complex in the ferric form has absorption maxima at 421 and 536 nm. In the ferrous form, this complex has absorption maxima at 442, 539, and 566 nm. The equilibrium constant for the binding of metyrapone by oxidized cytochrome P-450 is 2.3×108m−1and the binding is competitive with the substrate camphor. Metyrapone inhibits the uptake of oxygen by the coupled monooxygenase system probably by competing with camphor for the camphor-binding site on oxidized cytochrome P-450. The results presented indicate that the camphor-binding site is close to the oxygen-activating site on cytochrome P-450.