Effect of Reveromycin A on Experimental Tooth Movement in OPG-/- Mice
Effect of Reveromycin A on Experimental Tooth Movement in OPG-/- Mice
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DOI：
10.1177/0022034512451026
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发表时间：
2012-08-01
影响因子：
7.6
通讯作者：
Goto, S.
中科院分区：
文献类型：
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作者：
Tanaka, M.;Miyazawa, K.;Goto, S.
在骨保护素缺乏（OPG-/-）小鼠中，破骨细胞活性导致骨吸收超过骨形成，导致严重骨质疏松症的发展。因此，这种小鼠可用于研究骨质疏松症患者的牙槽骨。逆转霉素A（Reveromycin A，RM-A）是近年来发现的唯一一种作用于破骨细胞活化的药物。本研究旨在分析RM-A对OPG-/-小鼠（具有高水平骨转换的骨质疏松症患者模型）正畸治疗的影响。我们研究了OPG-/-和野生型（WT）小鼠在连续牙齿移动过程中的牙槽骨重建。用镍钛合金闭合弹簧施加正畸力移动上颌第一磨牙14 d。每日两次腹膜内施用RM-A钠盐（lmg/kg）。在OPG-/-小鼠中，牙齿移动距离更长，牙槽骨吸收增强，破骨细胞计数更大，血清碱性磷酸酶和抗酒石酸酸性磷酸酶水平相对于WT小鼠更高。然而，在OPG-/-小鼠中给予RM-A降低了这些参数。我们的结论是，RM-A正常化骨代谢和牙槽骨丢失在连续的牙齿移动OPG-/-小鼠。
In osteoprotegerin-deficient (OPG-/-) mice, osteoclast activity causes bone resorption to outpace bone formation, leading to the development of severe osteoporosis. Such mice are therefore useful for investigating the alveolar bone of patients with osteoporosis. Reveromycin A (RM-A) was recently identified as the unique agent acting on osteoclast activation. This study aimed to analyze the effect of RM-A on the orthodontic treatment of OPG-/- mice (a model of osteoporosis patients with high levels of bone turnover). We examined alveolar bone remodeling in OPG-/- and wild-type (WT) mice during continuous tooth movement. The orthodontic force was induced by means of a Ni-Ti closed-coil spring to move the maxillary first molar for 14 days. RM-A sodium salt (1 mg/kg) was administered intraperitoneally twice daily. In OPG-/- mice, the tooth movement distance was longer, alveolar bone resorption was enhanced, the osteoclast count was greater, and serum alkaline phosphatase and tartrate-resistant acid phosphatase levels were higher relative to those in WT mice. However, the administration of RM-A in OPG-/- mice reduced these parameters. We conclude that RM-A normalizes bone metabolism and loss of alveolar bone during continuous tooth movement in OPG-/- mice.