Regulation of gene expression with thyroid hormone in rats with myocardial infarction.
Regulation of gene expression with thyroid hormone in rats with myocardial infarction.
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DOI：
10.1371/journal.pone.0040161
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Gerdes AM
中科院分区：
文献类型：
--
作者：
Chen YF;Pottala JV;Weltman NY;Ge X;Savinova OV;Gerdes AM
大面积透壁性心肌梗死（MI）后左心室（LV）重构导致数百个基因表达改变。甲状腺激素（TH）改善MI后左室重构和心脏功能。然而，分子基础是未知的。结扎雌性SD大鼠冠状动脉左前降支建立MI模型。将大鼠分为以下组：（1）假MI，（2）MI，和（3）MI+T4治疗（T4颗粒3.3 mg，60天释放，MI后立即皮下植入）。手术后4周，从LV非梗塞区域分离总RNA，用于使用Illumina RatRef-12 Expression BeadChip Platform进行微阵列分析。在22，523个基因中检测到13，188个基因的信号，其中与Sham MI大鼠相比，MI大鼠中154个基因的表达降低，200个基因的表达增加（错误发现率（FDR）<0.05）。与MI大鼠相比，T4治疗降低了27个基因的表达，增加了28个基因的表达。特别是，6个基因下调MI和12个基因上调MI逆转T4。通过T4处理改变的55个基因中的大多数属于结合（24）和生物过程下的分子功能类别，生物过程包括免疫系统过程（9）、多生物体过程（5）和非排他性的生物调节（19）。这些结果表明，分子功能和生物学过程的基因表达的改变可能参与了甲状腺激素治疗大鼠MI后的有益作用。
The expression of hundreds of genes is altered in response to left ventricular (LV) remodeling following large transmural myocardial infarction (MI). Thyroid hormone (TH) improves LV remodeling and cardiac performance after MI. However, the molecular basis is unknown. MI was produced by ligation of the left anterior descending coronary artery in female SD rats. Rats were divided into the following groups: (1) Sham MI, (2) MI, and (3) MI+T4 treatment (T4 pellet 3.3 mg, 60 days release, implanted subcutaneously immediately following MI). Four weeks after surgery, total RNA was isolated from LV non-infarcted areas for microarray analysis using the Illumina RatRef-12 Expression BeadChip Platform. Signals were detected in 13,188 genes (out of 22,523), of which the expression of 154 genes were decreased and the expression of 200 genes were increased in MI rats compared with Sham MI rats (false discovery rate (FDR) <0.05). Compared to MI rats, T4 treatment decreased expression of 27 genes and increased expression of 28 genes. In particular, 6 genes down-regulated by MI and 12 genes up-regulated by MI were reversed by T4. Most of the 55 genes altered by T4 treatment are in the category of molecular function under binding (24) and biological processes which includes immune system process (9), multi-organism process (5) and biological regulation (19) nonexclusively. These results suggest that altered expression of genes for molecular function and biological process may be involved in the beneficial effects of thyroid hormone treatment following MI in rats.
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影响因子：
7
作者：
Kuhn, K;Baker, SC;Chee, MS
通讯作者：
Chee, MS
影响因子：
12.3
作者：
Gentleman RC;Carey VJ;Bates DM;Bolstad B;Dettling M;Dudoit S;Ellis B;Gautier L;Ge Y;Gentry J;Hornik K;Hothorn T;Huber W;Iacus S;Irizarry R;Leisch F;Li C;Maechler M;Rossini AJ;Sawitzki G;Smith C;Smyth G;Tierney L;Yang JY;Zhang J
通讯作者：
Zhang J
影响因子：
4.6
作者：
Deacon, Dekker C.;Nevis, Kathleen R.;Burns, Caroline E.
通讯作者：
Burns, Caroline E.
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y
影响因子：
56.9
作者：
Maisonpierre, PC;Suri, C;Yancopoulos, GD
通讯作者：
Yancopoulos, GD