The cultivation of cells from adult epidermis.
The cultivation of cells from adult epidermis.
复制标题
成人表皮细胞的培养。
DOI：
--
复制
发表时间：
1960
影响因子：
6.5
通讯作者：
C. Hooper
中科院分区：
文献类型：
--
作者：
C. Cruickshank;J. Cooper;C. Hooper
成人皮肤的培养以前已经通过两种主要方法完成。Medawar（1）首次使用并随后由Cruickshank（2）修改的表皮薄片与下层真皮一起漂浮在流体介质表面上，导致迁移活动且没有生长。主要由Pomerat及其同事（3，4）和Earle等人（5）开发的血浆凝块培养引起上皮细胞的生长，但成纤维细胞的过度生长是常见的。
The cultivation of adult skin has been previously accomplished by two main methods. The flotation of thin slices of epidermis together with the underlying dermis on the surface of a fluid medium, as first used by Medawar (1) and subsequently modified by Cruickshank (2), results in migratory activity and no outgrowth. Cultivation in plasma clots as exploited chiefly by Pomerat and his associates (3, 4) and by Earle et al. (5) gives rise to an outgrowth of epithelial cells but overgrowth by fibroblasts is common.