PURE CULTURE OF PHYSARUM POLYCEPHALUM ON A PARTIALLY DEFINED SOLUBLE MEDIUM
PURE CULTURE OF PHYSARUM POLYCEPHALUM ON A PARTIALLY DEFINED SOLUBLE MEDIUM
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DOI：
10.1099/00221287-25-1-47
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发表时间：
1961-01-01
期刊：
影响因子：
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通讯作者：
RUSCH, HP
中科院分区：
文献类型：
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作者：
DANIEL, JW;RUSCH, HP
从多头绒泡菌（Physarumpolycephalum）中分离到一株多核原质黏菌，在含有1%（w/v）胰蛋白胨、1%（w/v）葡萄糖、0.15%（w/v）酵母膏、0.3%（w/v）CaCO_3、无机盐和少量鸡胚提取物的培养基上生长。在这种培养基中，微生物既可以作为单个大原质团在表面培养中生长，也可以作为微小原质团的悬浮液在浸没培养中生长。从1 ml初始接种物开始。在3天的培养物中，在72小时时，浸没培养物中的平均疟原虫产量为约80 mg干重/20 ml培养基。生长仅在存在少量未鉴定因子的情况下发生，该因子尤其存在于鸡胚提取物和胎牛血清中。一株多头拟单胞菌在此培养基上连续生长4年以上，生长速率无明显下降。在适当的条件下，来自振荡培养物的微小原质团悬浮液将融合形成单个大的表面原质团，其表现出同步有丝分裂。
A wild strain of the multinucleate plasmodial myxomycete Physarum poly-cephalum was isolated in pure culture and grown on a medium consisting of 1% (w/v) Tryptone, 1% (w/v) glucose, 0.15% (w/v) yeast extract, 0.3% (w/v) CaCO3, inorganic salts and a small amount of chick embryo extract. The organism may be grown with this medium either as a single large plasmodium on surface culture, or as a suspension of tiny plasmodia in submerged culture. From an initial inoculum of 1 ml. of a 3-day culture, the average plasmodial yield in a submerged culture was about 80 mg dry weight/20 ml medium at 72 hours. Growth occurred only in the presence of small amounts of an unidentified factor which was present particularly in chick embryo extract and fetal calf serum. An isolate of P. polycephalum was grown continuously on this medium for over 4 years without an appreciable decrease in growth rate. Under proper conditions a suspension of tiny plasmodia from shaken culture will fuse to form a single large surface plasmodium which exhibits synchronous mitosis.