Cloning and characterization of the Arabidopsis thaliana lupeol synthase gene
Cloning and characterization of the Arabidopsis thaliana lupeol synthase gene
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DOI：
10.1016/s0031-9422(98)00366-5
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发表时间：
1998-12-01
期刊：
影响因子：
3.8
通讯作者：
Matsuda, SPT
中科院分区：
文献类型：
--
作者：
Herrera, JBR;Bartel, B;Matsuda, SPT
从拟南芥中分离到一个2274 bp的cDNA，其编码的蛋白质与来自同一生物体的环阿屯醇合酶具有57%的同源性。表达的重组蛋白编码羽扇豆醇合成酶，该合成酶将氧化角鲨烯转化为三萜羽扇豆醇作为主要产物。羽扇豆醇合酶是一种多功能酶，其形成其他三萜醇，包括β-香树脂醇，作为次要产物。序列分析表明，羽扇豆醇合酶是在植物与真菌和动物分化之后，从环阿屯醇合酶分化而来的。这种进化顺序是真菌和动物不产生羽扇豆醇的原因。(C)1998爱思唯尔科技有限公司版权所有。
A 2274bp Arabidopsis thaliana cDNA was isolated that encodes a protein 57% identical to cycloartenol synthase from the same organism. The expressed recombinant protein encodes lupeol synthase, which converts oxidosqualene to the triterpene lupeol as the major product. Lupeol synthase is a multifunctional enzyme that forms other triterpene alcohols, including beta-amyrin, as minor products. Sequence analysis suggests that lupeol synthase diverged from cycloartenol synthase after plants diverged from fungi and animals. This evolutionary order is the reason that fungi and animals do not make lupeol. (C) 1998 Elsevier Science Ltd. All rights reserved.