Development of ultrafast PCR assays for the event-specific detection of eleven approved genetically modified canola events in South Korea.
Development of ultrafast PCR assays for the event-specific detection of eleven approved genetically modified canola events in South Korea.
复制标题
开发超快速 PCR 检测方法，用于对韩国批准的 11 项转基因油菜籽事件进行事件特异性检测。
DOI：
10.1016/j.foodchem.2021.131419
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发表时间：
2021
期刊：
影响因子：
8.8
通讯作者：
Hyun
中科院分区：
文献类型：
--
作者：
Jieun Park;Do;Hong;Mi;Hae;Hyun
在韩国，来自11个单一事件的转基因（GM）油菜籽事件已被授权用于食品和饲料，但不用于种植。因此，开发一种快速准确的现场检测方法对于管理这些已批准的转基因油菜事件至关重要。在这项研究中，超快速聚合酶链反应（PCR）检测事件特异性检测11转基因油菜事件。使用超快速PCR对基于DNA和基于粉末的转基因油菜样品的检测限（LOD）范围为0.1%至0.01%，而这些超快速PCR检测的定量分析表明，相关系数（R2）范围为0.98至0.9903。这些结果表明，开发的检测方法可能具有足够的特异性和LOD能力，以检测11个特定的转基因油菜事件的伴随管理和监测，从而防止转基因油菜污染自然环境。
In Korea, genetically modified (GM) canola events derived from eleven single events have been authorized for food and feed, but not for cultivation. Therefore, the development of a rapid and accurate on-site detection method is crucial for the management of these approved GM canola events. In this study, ultrafast polymerase chain reaction (PCR) assays for the event-specific detection of eleven GM canola events were developed. The limit of detection (LOD) on DNA-based and powder-based GM canola samples of each primer set using the ultrafast PCR ranged from 0.1% to 0.01%, while the quantitative analysis of these ultrafast PCR assays, indicated that the correlation coefficient (R2) ranged from 0.98 to 0.9903. These results indicate that the developed assays may have sufficient specificity and LOD capacity to detect the eleven specific GM canola events for the attendant management and monitoring, thus preventing GM canola from contaminating the natural environment.