Multilayer microfluidic PEGDA hydrogels
Multilayer microfluidic PEGDA hydrogels
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DOI：
10.1016/j.biomaterials.2010.03.031
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发表时间：
2010-07-01
期刊：
影响因子：
14
通讯作者：
West, Jennifer L.
中科院分区：
文献类型：
--
作者：
Cuchiara, Michael P.;Allen, Alicia C. B.;West, Jennifer L.
体外稳健的3D组织类似物的开发受到被动的限制。扩散传质灌注微流控组织工程支架有望改善质量传输限制，促进复杂的，代谢密集的，临床相关的组织的发展。我们报告了一种简单而强大的多层复制模塑技术，其中聚（二甲基硅氧烷）（PDMS）和聚（乙二醇）二丙烯酸酯（PEGDA）连续复制模塑开发微流体PEGDA水凝胶网络嵌入在独立制造的PDMS外壳。我们证明了控制溶质-支架有效扩散率作为溶质分子量和水凝胶浓度的函数的能力。在载有细胞的微流体水凝胶中，我们证明了与静态对照相比，灌注水凝胶系统中的细胞活力增加。我们观察到，在距离灌注通道最多1 mm处，在所有时间点细胞活力均显著增加，大于零。时空质量运输和细胞活力梯度的知识提供了必要的有用的工程设计参数，以最大限度地提高整体支架的活力和代谢密度。这项工作在水凝胶的开发中具有应用，作为体外诊断，并最终作为基于再生医学的治疗方法。(c)2010爱思唯尔有限公司版权所有。
Development of robust 3D tissue analogs in vitro is limited by passive. diffusional mass transport. Perfused microfluidic tissue engineering scaffolds hold the promise to improve mass transport limitations and promote the development of complex, metabolically dense, and clinically relevant tissues. We report a simple and robust multilayer replica molding technique in which poly(dimethylsiloxane) (PDMS) and poly(ethylene glycol) diacrylate (PEGDA) are serially replica molded to develop microfluidic PEGDA hydrogel networks embedded within independently fabricated PDMS housings. We demonstrate the ability to control solute-scaffold effective diffusivity as a function of solute molecular weight and hydrogel concentration. Within cell laden microfluidic hydrogels, we demonstrate increased cellular viability in perfused hydrogel systems compared to static controls. We observed a significant increase in cell viability at all time points greater than zero at distances up to 1 mm from the perfused channel. Knowledge of spatiotemporal mass transport and cell viability gradients provides useful engineering design parameters necessary to maximize overall scaffold viability and metabolic density. This work has applications in the development of hydrogels as in vitro diagnostics and ultimately as regenerative medicine based therapeutics. (c) 2010 Elsevier Ltd. All rights reserved.