Proton conductivity of glycosaminoglycans
Proton conductivity of glycosaminoglycans
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DOI：
10.1371/journal.pone.0202713
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发表时间：
2019-03-08
期刊：
影响因子：
3.7
通讯作者：
Rolandi, Marco
中科院分区：
文献类型：
--
作者：
Selberg, John;Jia, Manping;Rolandi, Marco
质子导电性在许多自然现象中是重要的，包括线粒体和古细菌中的氧化磷酸化，抗生素短杆菌肽的解偶联膜电位，以及甲藻中的质子驱动的生物发光。在所有这些现象中，质子的传导沿着水和亲水残基之间的氢键链发生。这些氢键链也存在于许多水合生物聚合物和大分子中，包括胶原蛋白、角蛋白、壳聚糖和各种蛋白质如反射蛋白。所有这些材料也都是质子导体。最近，我们的研究小组发现，在鲨鱼的电感应器官--洛仑兹尼壶腹中发现的胶状物，是天然存在的最高质子传导物质。凝胶具有复杂的组成，但我们提出导电性是由于硫酸糖胺聚糖角质素（KS）。在这里，我们测量从牛角膜纯化的水合硫酸角质素的质子传导率。PdHx在0.50 +/- 0.11 mS cm（-1）处接触，这与Lorenzini胶壶腹在2 +/- 1 mS cm（-1）处的接触一致。质子电导率，尽管具有较低的值，也被具有类似化学结构的其他糖胺聚糖共享，包括硫酸皮肤素、硫酸软骨素A、硫酸乙酰肝素和透明质酸。这一观察结果支持了质子传导性与生物聚合物化学结构之间的关系。
Proton conductivity is important in many natural phenomena including oxidative phosphorylation in mitochondria and archaea, uncoupling membrane potentials by the antibiotic Gramicidin, and proton actuated bioluminescence in dinoflagellate. In all of these phenomena, the conduction of protons occurs along chains of hydrogen bonds between water and hydrophilic residues. These chains of hydrogen bonds are also present in many hydrated biopolymers and macromolecule including collagen, keratin, chitosan, and various proteins such as reflectin. All of these materials are also proton conductors. Recently, our group has discovered that the jelly found in the Ampullae of Lorenzini-shark's electro-sensing organs-is the highest naturally occurring proton conducting substance. The jelly has a complex composition, but we proposed that the conductivity is due to the glycosaminoglycan keratan sulfate (KS). Here we measure the proton conductivity of hydrated keratan sulfate purified from Bovine Cornea. PdHx contacts at 0.50 +/- 0.11 mS cm(-1), which is consistent to that of Ampullae of Lorenzini jelly at 2 +/- 1 mS cm(-1). Proton conductivity, albeit with lower values, is also shared by other glycosaminoglycans with similar chemical structures including dermatan sulfate, chondroitin sulfate A, heparan sulfate, and hyaluronic acid. This observation supports the relationship between proton conductivity and the chemical structure of biopolymers.