Genetic recombination after cell fusion of protoplasts from the facultative alkaliphile Bacillus sp. C-125
Genetic recombination after cell fusion of protoplasts from the facultative alkaliphile Bacillus sp. C-125
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兼性嗜碱性芽孢杆菌原生质体细胞融合后的基因重组。
DOI：
10.1099/13500872-140-11-3085
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发表时间：
1994
期刊：
影响因子：
1.5
通讯作者：
K. Horikoshi
中科院分区：
文献类型：
--
作者：
R. Aono;M. Ito;K. Joblin;K. Horikoshi
通过用溶菌酶处理，从兼性嗜碱芽孢杆菌C-125的两个营养缺陷型和耐缺氧菌株（分别为Met-Nalrand Thr-Strr）制备原生质体。原生质体在聚乙二醇存在下有效融合。两个亲本原生质体之间获得的融合子再生固体培养基上含有两种抗生素，萘啶酸（Nal）和链霉素（Str）。亲本原生质体在非选择性培养基上以高频率（43-97%）再生，但在选择性培养基上不再生。NalrStrrfusants具有杆菌的形式。Met和Thr标记在具有主要Met+Thr+表型的融合子之间分离。离合体似乎恢复了亲本的倍性。
Protoplasts were prepared from two auxotrophic and antibiotic-resistant strains (Met-Nalrand Thr-Strr, respectively) of the facultative alkaliphile Bacillus sp. C-125 by treatment with lysozyme. The protoplasts fused effectively in the presence of polyethylene glycol. Fusants obtained between two parental protoplasts were regenerated on solid medium containing the two antibiotics, nalidixic acid (Nal) and streptomycin (Str). Parental protoplasts were regenerated at high frequency (43-97%) on non-selective medium but not on selective medium. The NalrStrrfusants had the form of bacilli. Met and Thr markers segregated among the fusants with a predominantly Met+Thr+phenotype. The exfusants seemed to restore the parental ploidy.