Molecular characterization and functional analysis of the GATA4 in tongue sole (Cynoglossus semilaevis)
Molecular characterization and functional analysis of the GATA4 in tongue sole (Cynoglossus semilaevis)
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舌鳎 (Cynoglossus semilaevis) GATA4 的分子特征和功能分析
DOI：
10.1016/j.cbpb.2015.12.001
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发表时间：
2016-03-01
影响因子：
2.2
通讯作者：
He, Yan
中科院分区：
文献类型：
--
作者：
Liu, Jinxiang;Zhang, Wei;He, Yan
转录因子的GATA家族具有两个锌指域的特征，并参与了不同的细胞过程。 GATA4是一个高度保守的转录因子，可调节胚胎形态发生和细胞分化。脊椎动物中的GATA4调节其靶基因以影响生殖脊分化。在这项研究中，从舌鞋底（Cynoglossus semilaevis）中的GATA4被表征以了解该转录因子在性别分化中的功能。 C. semilaevis gata4的全长cDNA包含2031 bp，编码了一个预测的多肽，该多肽由402个氨基酸组成，带有两个保守的锌指域。系统发育，基因结构和同义分析表明，c emilaevis gata4与四足动物GATA4同源。 c emilaevis gata4的mRNA转录物在心脏，肝脏和性腺中表现出很高的表达。 GATA4表达在男性和雌性性腺中是二态的。胚胎发育表达谱揭示了c半evis gata4可能参与形态发生。原位杂交结果表明，睾丸的精子和精子细胞以及卵巢的原发性卵母细胞和二次卵母细胞中的精子中有强的GATA4信号。在三种性别中，两个CpG位点（-2738和2647）的雄性，伪男性和雌性性腺中c emiLaevis gata4的表达显着不同。根据这些结果，我们推测GATA4在性别差异中起潜在的作用。这项研究为在c emilaevis的进一步控制育种技术奠定了基础。 （c）2015 Elsevier Inc.保留所有权利。
The GATA family of transcription factors is characterized by two zinc finger domains and is involved in different cellular processes. GATA4 is a highly conserved transcription factor that regulates embryonic morphogenesis and cellular differentiation. GATA4 in vertebrates regulates its target genes to influence genital ridge differentiation. In this study, the GATA4 from tongue sole (Cynoglossus semilaevis) was characterized to understand the function of this transcription factor in sex differentiation. The full-length cDNA of C. semilaevis GATA4 comprised 2031 bp, encoding a predicted polypeptide consisting of 402 amino acids with two conserved zinc finger domains. Phylogenetic, gene structure, and synteny analyses showed that C semilaevis GATA4 was homologous to tetrapod GATA4. The mRNA transcript of C semilaevis GATA4 exhibited high expression in the heart, liver, and gonad. GATA4 expression is dimorphic in the male and female gonads. Embryonic development expression profiles revealed the possible involvement of C semilaevis GATA4 in morphogenesis. In situ hybridization results showed strong GATA4 signals in the spermatogonia and spermatocytes of the testis and in the oogonia, primary oocytes, and secondary oocytes of the ovary. The expression of C semilaevis GATA4 in the male, pseudomale, and female gonads showed significantly different methylation levels of the two CpG sites (-2738 and 2647) among the three genders. Basing on these results, we speculated that GATA4 plays a potential role in sex differentiation. This study lays the groundwork for further sex control breeding techniques in C semilaevis. (C) 2015 Elsevier Inc. All rights reserved.