Elcatonin Injections Suppress Systemic Bone Resorption without Affecting Cortical Bone Regeneration after Drill-Hole Injuries in Mice
Elcatonin Injections Suppress Systemic Bone Resorption without Affecting Cortical Bone Regeneration after Drill-Hole Injuries in Mice
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DOI：
10.1002/jor.20920
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发表时间：
2009-12-01
影响因子：
2.8
通讯作者：
Nakamura, Toshitaka
中科院分区：
文献类型：
--
作者：
Katae, Yuji;Tanaka, Shinya;Nakamura, Toshitaka
假设在骨折愈合过程中，矿物质代谢发生了全身性变化，这可能导致既往骨折患者发生继发性骨折的倾向。对骨质疏松性骨折患者的初步治疗对于预防因骨和肌肉萎缩以及继发性骨折而导致的日常生活残疾非常重要，尽管骨折愈合期间的全身和局部骨代谢尚未得到很好的了解。我们评估了骨损伤和注射降钙素作为初始治疗对全身和局部骨转换和骨伤口愈合的影响。在12周龄雄性C57BL/6J小鼠左侧股骨和胫骨的骨干上钻两个孔。在为期28天的实验结束之前，他们每周三次接受三次依卡托宁或一种载体的治疗。在赋形剂治疗的小鼠中，尿I型胶原C-端肽(CTX)增加，腰椎骨密度(BMD)降低。灯盏花素可预防尿CTX升高和BMDS的降低。在未损伤的股骨干骺端，破骨细胞表面增加到第28天，而降钙素则抑制它。在骨折部位，依卡托宁促进了成骨细胞的增殖，并没有延迟骨缺损的愈合。骨损伤促进了全身和局部的骨转换，注射依降钙素抑制了全身骨吸收的加速，而不延迟在骨缺损处填充再生的皮质骨。(C)2009年骨科研究会。由Wiley期刊公司出版，J Orthop Res 27：1652-1658,2009
It is assumed that there are systemic changes in mineral metabolism during fracture healing that may cause a predisposition to sequential fractures in osteoporotic patients who suffered from previous fractures. Initial therapies for patients with osteoporotic fractures are important to prevent disabilities in daily life consequent to bone and muscle atrophies, and sequential fractures, although systemic and local bone metabolism during fracture healing have not been well understood. We evaluated the effects of bone injury and elcatonin injection as an initial therapy on systemic and local bone turnover and bone wound healing. Two drill holes were made in the diaphysis of the left femur and tibia of 12-week-old male C57BL/6J mice. They were treated with three doses of elcatonin or a vehicle thrice a week until the end of the 28-day experiment. Urinary crosslinked C-telopeptide of type I collagen (CTX) increased and the bone mineral densities (BMDs) in the lumbar vertebrae decreased in the vehicle-treated mice. Elcatonin injection prevented increases in urinary CTX and reduction of the BMDs. In the noninjured femoral metaphysis, osteoclast surface increased until day 28, whereas elcatonin suppressed it. In the fracture site, elcatonin facilitated osteoblast proliferation and did not delay the healing of the bone defect. Bone injuries accelerated bone turnover systemically and locally, and the elcatonin injections suppressed the systemic acceleration of bone resorption without a delay of filling regenerated cortical bone in the bone defect. (C) 2009 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 27:1652-1658, 2009