Biological identifications through DNA barcodes
Biological identifications through DNA barcodes
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DOI：
10.1098/rspb.2002.2218
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发表时间：
2003-02-07
影响因子：
4.7
通讯作者：
DeWaard, JR
中科院分区：
文献类型：
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作者：
Hebert, PDN;Cywinska, A;DeWaard, JR
尽管许多生物学研究依赖于物种诊断，但分类学专业知识正在崩溃。我们相信，可持续鉴定能力的唯一前景在于构建使用DNA序列作为分类单元“条形码”的系统。我们建立了线粒体基因细胞色素c氧化酶I（COI）可以作为一个全球生物识别系统的动物的核心。首先，我们证明了COI配置文件，来自高分类类别的低密度采样，通常分配新分析的分类群到适当的门或顺序。其次，我们证明了物种级别的分配可以通过创建全面的COI配置文件。一个模型COI配置文件，分析的基础上，从每一个200个密切相关的鳞翅目物种的单个个体，是100%成功地正确识别随后的标本。一旦充分开发，COI识别系统将为当前的物种识别问题提供可靠、具有成本效益和可获得的解决方案。它的组装也将产生对生命多样性和分子进化规则的重要新见解。
Although much biological research depends upon species diagnoses, taxonomic expertise is collapsing. We are convinced that the sole prospect for a sustainable identification capability lies in the construction of systems that employ DNA sequences as taxon 'barcodes'. We establish that the mitochondrial gene cytochrome c oxidase I (COI) can serve as the core of a global bioidentification system for animals. First, we demonstrate that COI profiles, derived from the low-density sampling of higher taxonomic categories, ordinarily assign newly analysed taxa to the appropriate phylum or order. Second, we demonstrate that species-level assignments can be obtained by creating comprehensive COI profiles. A model COI profile, based upon the analysis of a single individual from each of 200 closely allied species of lepidopterans, was 100% successful in correctly identifying subsequent specimens. When fully developed, a COI identification system will provide a reliable, cost-effective and accessible solution to the current problem of species identification. Its assembly will also generate important new insights into the diversification of life and the rules of molecular evolution.