Evaluation of urinary metabolites of 1-nitropyrene as biomarkers for exposure to diesel exhaust in taxi drivers of Shenyang, China
Evaluation of urinary metabolites of 1-nitropyrene as biomarkers for exposure to diesel exhaust in taxi drivers of Shenyang, China
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DOI：
10.1038/jes.2012.40
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发表时间：
2013-03-01
影响因子：
4.5
通讯作者：
Simpson, Christopher D.
中科院分区：
文献类型：
--
作者：
Miller-Schulze, Justin P.;Paulsen, Michael;Simpson, Christopher D.
柴油机尾气(DE)是颗粒物(PM)毒性的重要贡献者。1-硝基吡喃(1-NP)已被用作DE的分子标志物，1-NP的尿代谢物被认为是DE暴露的生物标志物。在这项研究中，评估了几种尿中1-NP代谢物作为短期DE暴露标志物的有效性。研究人群是来自沈阳的24名出租车司机中国，他们提交了在轮班前、之后和第二天早上收集的尿样。所研究的尿代谢物为羟基-1-硝基-1-吡喃(3-，6-，8-OHNPs)和羟基-N-乙酰基-1-氨基比林(3-，6-，8-OHNAAP)的异构体。根据司机轮班期间和轮班后采集的空气样本中1-NP的暴露情况，估计DE的暴露情况；在司机的尿液中持续检测到6-和8-OHNP，以及8-OHNAAP。出租车司机尿液中的代谢物浓度高于先前在非职业暴露受试者中报告的代谢物水平；然而，在特定受试者暴露于1-NP与轮班结束时或第二天早上空闲时测得的尿代谢物之间没有观察到关联。在连续的尿样中观察到显著的代谢物水平的自相关，由此估计尿液消除的半衰期类似于10-12小时。这些观察表明，在职业环境中，尿1-NP代谢物可能更适合作为持续暴露的标志(几天的时间尺度)，而不是与单一轮班相关的急性暴露的指示物。《暴露科学与环境流行病学杂志》(2013年)23期，第170-175期；DOI：10.1038/jes.2012.40；2012年5月16日在线发布
Diesel exhaust (DE) is a significant contributor to the toxicity associated with particulate matter (PM). 1-Nitropyrene (1-NP) has been used as a molecular marker for DE, and the urinary metabolites of 1-NP have been proposed as biomarkers for exposure to DE. In this study, several urinary 1-NP metabolites were evaluated for their utility as markers of short-term exposures to DE. The study population was a cohort of 24 taxi drivers from Shenyang, China, who submitted urine samples collected before, after, and the next morning following their workshifts. The urinary metabolites studied were isomers of hydroxy-1-nitropyrene (3-, 6-, 8- OHNPs) and hydroxy-N-acetyl-1-aminopyrene (3-,6-, 8-OHNAAPs). Exposure to DE was estimated based on exposure to 1-NP in air samples collected during and after the driver's workshift; 6- and 8-OHNP, and 8-OHNAAP were consistently detected in the drivers' urine. Concentrations of the metabolites in the taxi drivers' urine were greater than metabolite levels previously reported in non-occupationally exposed subjects; however no associations were observed between subject-specific exposures to 1-NP and urinary metabolites measured at the end of the workshift or in the next morning void. Significant autocorrelation was observed in metabolite levels in successive urine samples, from which half-lives for urinary elimination of similar to 10-12 h were estimated. These observations suggest that, in an occupational setting, urinary 1-NP metabolites may be more suitable as markers of ongoing exposure (timescales of several days) rather than indicators of acute exposure associated with single workshifts. Journal of Exposure Science and Environmental Epidemiology (2013) 23, 170-175; doi:10.1038/jes.2012.40; published online 16 May 2012