IMMORTALIZATION OF MONKEY EPITHELIAL-CELLS BY SPECIFIC FRAGMENTS OF EPSTEIN-BARR VIRUS-DNA
IMMORTALIZATION OF MONKEY EPITHELIAL-CELLS BY SPECIFIC FRAGMENTS OF EPSTEIN-BARR VIRUS-DNA
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DOI：
10.1038/309078a0
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发表时间：
1984-01-01
期刊：
影响因子：
64.8
通讯作者：
KARRAN, L
中科院分区：
文献类型：
--
作者：
GRIFFIN, BE;KARRAN, L
EB病毒（EBV）是DNA肿瘤病毒中的独特病毒，因为其与两种人类恶性肿瘤，伯基特淋巴瘤和鼻咽癌（NPC）相关，前者是B淋巴细胞的肿瘤，后者包括鼻咽的低分化上皮细胞1 -4。病毒基因产物尚未明确与这些恶性疾病相关，尽管EBV核抗原（EBNA）似乎在EBV感染的细胞中无处不在;事实上，通过免疫荧光检测EBNA通常被视为病毒基因组存在的指示5，6。作为研究EBV基因组的哪一部分负责转化以及在B淋巴细胞和上皮细胞的情况下是否涉及相同的细胞转化机制的研究的一部分，我们试图确定是否可以通过EBV基因组的特定区域的存在在灵长类上皮细胞中诱导可检测的细胞改变。我们在这里报告说，它可以-结果是细胞的永生化。
Epstein–Barr virus (EBV) is unique among the DNA tumour viruses by virtue of its association with two human malignancies, Burkitt's lymphoma and nasopharyngeal carcinoma (NPC), the former a tumour of B lymphocytes and the latter encompassing low-differentiated epithelial cells of the nasopharynx1–4. A viral gene product has not been definitively linked to these malignant diseases, although an EBV nuclear antigen(s) (EBNA) seems to be ubiquitous in EBV infected cells; indeed, the detection of EBNA by immunofluorescence is often taken as an indication of the presence of the viral genome5,6. As part of a study to investigate which part of the EBV genome is responsible for transformation and whether the same mechanism of cellular transformation is involved in the case of B lymphocytes and epithelial cells, we have tried to establish whether a detectable cellular alteration(s) can be induced in primate epithelial cells by the presence of a specific region of the EBV genome. We report here that it can—the result is immortalization of the cells.