Metabolites from the fungal pathogen Batrachochytrium dendrobatidis (bd) reduce Bd load in Cuban treefrog tadpoles
Metabolites from the fungal pathogen Batrachochytrium dendrobatidis (bd) reduce Bd load in Cuban treefrog tadpoles
复制标题
真菌病原体 Batrachochytrium dendrobatidis (bd) 的代谢物可减少古巴树蛙蝌蚪中的 Bd 负荷
DOI：
10.1111/1365-2664.14242
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发表时间：
2022
影响因子：
5.7
通讯作者：
McMahon, Taegan A.
中科院分区：
文献类型：
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作者：
Nordheim, Caitlin L.;Detmering, Sarah E.;Civitello, David J.;Johnson, Pieter T. J.;Rohr, Jason R.;McMahon, Taegan A.
树突巴氏杆菌(Batrachochytrium Dendrobatidis，Bd)与全球范围内两栖动物数量的大量下降有关。野生动物疫苗接种运动已被证明在减轻来自其他病原体的损害方面是有效的，有证据表明，成年青蛙在暴露于被杀死的Bd游动孢子及其产生的代谢物时，可以获得对Bd的抵抗力。在这里，我们调查了古巴树蛙蝌蚪是否可以通过暴露于由被杀死的游动孢子或可溶性Bd代谢物组成的预防性治疗来获得对Bd的保护。我们采用2 × 2析因设计，将游动孢子杀灭与否与Bd代谢物的存在与否进行交叉。所有宿主随后都暴露在活的Bd中以评估易感性。暴露于被杀死的游动孢子没有诱导保护性反应。然而，暴露于Bd代谢物的蝌蚪的Bd强度和患病率显著低于未暴露于Bd代谢物的蝌蚪。Bd产生的代谢物不会造成Bd感染的风险，因此是一种流行病学安全的预防治疗，保护蝌蚪免受Bd的侵害。这项工作为保护野生两栖动物作为控制Bd相关下降的疾病管理策略提供了一个很有前途的潜力。
Batrachochytrium dendrobatidis(Bd) has been associated with massive amphibian population declines worldwide. Wildlife vaccination campaigns have proven effective for mitigating damage from other pathogens, and there is evidence that adult frogs can acquire resistance to Bd when exposed to killed Bd zoospores and the metabolites they produced.Here, we investigated whether Cuban treefrogs tadpolesOsteopilus septentrionaliscan gain protection from Bd through exposure to a prophylaxis treatment composed of killed zoospores or soluble Bd metabolites. We used a 2 × 2 factorial design, crossing the presence or absence of killed zoospores with the presence or absence of Bd metabolites. All hosts were subsequently exposed to live Bd to evaluate susceptibility.Exposure to killed zoospores did not induce a protective response. However, tadpoles exposed to Bd metabolites had significantly lower Bd intensity and prevalence than tadpoles that were not exposed to metabolites.The metabolites Bd produce pose no risk of Bd infection and therefore make an epidemiologically safe prophylaxis treatment, protecting tadpoles against Bd. This work provides a promising potential for protecting amphibians in the wild as a disease management strategy for controlling Bd‐associated declines.