Carbon monoxide-dependent transcriptional changes in a thermophilic, carbon monoxide-utilizing, hydrogen-evolving bacterium Calderihabitans maritimus KKC1 revealed by transcriptomic analysis
Carbon monoxide-dependent transcriptional changes in a thermophilic, carbon monoxide-utilizing, hydrogen-evolving bacterium Calderihabitans maritimus KKC1 revealed by transcriptomic analysis
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DOI：
10.1007/s00792-020-01175-z
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发表时间：
2020-05-09
期刊：
影响因子：
2.9
通讯作者：
Sako, Yoshihiko
中科院分区：
文献类型：
--
作者：
Inoue, Masao;Izumihara, Hikaru;Sako, Yoshihiko
Calderihabitans maritimus KKC 1是一种嗜热的、利用一氧化碳（CO）的、产氢的细菌，其具有7个用于厌氧CO氧化酶的cooS基因和6个用于[NiFe]氢化酶的hyd基因，并且能够使用多种与CO氧化偶联的电子受体。为了了解这些独特的特征和生物体对CO的转录适应之间的关系，我们对C. maritimus KKC 1在100% CO和N-2条件下生长。在其3114个基因中，58个和32个基因在CO存在下分别显著上调和下调。一个cooS-ech基因簇，一个“孤儿”cooS基因，双向hyd基因上调下CO，而氢摄取hyd基因下调。厌氧呼吸基因的转录变化支持了电子受体在C. maritimus KKC 1的CO代谢。总的来说，大多数差异表达的基因是氧化还原酶样基因，表明代谢适应CO氧化后细胞的氧化还原变化。此外，我们的研究结果表明，一氧化碳的转录反应机制，涉及多个转录因子，以及一氧化碳响应转录激活因子（CooA）。我们的研究结果揭示了不同的机制，转录和代谢适应CO在CO利用和氢进化细菌。
Calderihabitans maritimus KKC1 is a thermophilic, carbon monoxide (CO)-utilizing, hydrogen-evolving bacterium that harbors seven cooS genes for anaerobic CO dehydrogenases and six hyd genes for [NiFe] hydrogenases and capable of using a variety of electron acceptors coupled to CO oxidation. To understand the relationships among these unique features and the transcriptional adaptation of the organism to CO, we performed a transcriptome analysis of C. maritimus KKC1 grown under 100% CO and N-2 conditions. Of its 3114 genes, 58 and 32 genes were significantly upregulated and downregulated in the presence of CO, respectively. A cooS-ech gene cluster, an "orphan" cooS gene, and bidirectional hyd genes were upregulated under CO, whereas hydrogen-uptake hyd genes were downregulated. Transcriptional changes in anaerobic respiratory genes supported the broad usage of electron acceptors in C. maritimus KKC1 under CO metabolism. Overall, the majority of the differentially expressed genes were oxidoreductase-like genes, suggesting metabolic adaptation to the cellular redox change upon CO oxidation. Moreover, our results suggest a transcriptional response mechanism to CO that involves multiple transcription factors, as well as a CO-responsive transcriptional activator (CooA). Our findings shed light on the diverse mechanisms for transcriptional and metabolic adaptations to CO in CO-utilizing and hydrogen-evolving bacteria.