Comparative study on different detection methods of superoxide radicals in plant tissues
Comparative study on different detection methods of superoxide radicals in plant tissues
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DOI：
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发表时间：
2012
影响因子：
4.3
通讯作者：
Qinying Wang
中科院分区：
文献类型：
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作者：
Qinying Wang
用电子顺磁共振自旋捕捉技术、羟胺氧化法、二氢乙锭荧光探针法和硝基蓝四氮唑原位定位法研究了Cd胁迫下水稻幼苗根和叶中超氧阴离子自由基(O·-2)的变化。结果表明，外源Cd浓度在0~60 mg·L-1范围内，随着外源Cd浓度的增加，超氧阴离子自由基(O·-2)的生成量增加，之后又呈下降趋势。4种方法的结果基本一致，但前两种方法更适合于定量，后两种方法只能反映O·-2的产生趋势这些方法可以选择性地揭示环境污染物对植物组织中O·-2产生的影响。
Changes of superoxide anion radicals(O·-2) in roots and leaves of rice seedlings exposed to Cd for two days were investigated using electron paramagnetic resonance(EPR) spin trapping technique,hydroxylamine oxidation method,dihydroethidium(DHE) fluorescent probing method as well as nitroblue tetrazolium(NBT) in situ localization,respectively.The results showed that O·-2 production increased with the increase of extraneous Cd from 0 to 60 mg· L-1,followed by a decline hereafter.The four methods showed consistent results in general while the former two methods are more suitable for quantification,and the latter two ones can only show the trend of O·-2 production.Thus,these methods can be used selectively to reveal the change of O·-2 production in plant tissues exposed to environmental pollutants.