Systemic modulation of serotonergic synapses via reuptake blockade or 5HT1A receptor antagonism does not alter perithreshold taste sensitivity in rats.
Systemic modulation of serotonergic synapses via reuptake blockade or 5HT1A receptor antagonism does not alter perithreshold taste sensitivity in rats.
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DOI：
10.1093/chemse/bju028
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发表时间：
2014-09
期刊：
影响因子：
3.5
通讯作者：
C. Mathes;A. Spector
中科院分区：
文献类型：
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作者：
C. Mathes;A. Spector
帕罗西汀对5-羟色胺（5-HT）再摄取的全身性阻断已被证明可增加人体对蔗糖和奎宁的敏感性。在这里，使用2响应操作性味觉检测任务，我们测量了帕罗西汀和5 HT 1A受体拮抗剂WAY 100635对大鼠区分蔗糖，NaCl和柠檬酸的能力的影响。在建立个体心理测量函数后，选择5种浓度的每种味觉刺激来代表浓度-反应曲线的动态部分，并且在给予载体、帕罗西汀（腹膜内7 mg/kg）和WAY 100635（皮下0.3mg/kg;静脉内1 mg/kg）后评估大鼠对这些刺激的表现。尽管有时各浓度的总体性能从溶剂至药物条件最多下降5%，但给药后在心理测量功能参数（渐近线、斜率或EC 50）上未观察到相对于溶剂的差异。在人类中的研究结果相反，我们的研究结果表明，调制的5 HT活性几乎没有影响蔗糖检测在大鼠的阈上浓度，至少在这项任务中使用的剂量。在大鼠模型中，味蕾中5-羟色胺的所谓旁分泌/神经分泌作用可能以不影响明显味觉检测行为的方式起作用。
Systemic blockade of serotonin (5HT) reuptake with paroxetine has been shown to increase sensitivity to sucrose and quinine in humans. Here, using a 2-response operant taste detection task, we measured the effect of paroxetine and the 5HT1A receptor antagonist WAY100635 on the ability of rats to discriminate sucrose, NaCl, and citric acid from water. After establishing individual psychometric functions, 5 concentrations of each taste stimulus were chosen to represent the dynamic portion of the concentration-response curve, and the performance of the rats to these stimuli was assessed after vehicle, paroxetine (7mg/kg intraperitoneally), and WAY100635 (0.3mg/kg subcutaneously; 1mg/kg intravenously) administration. Although, at times, overall performance across concentrations dropped, at most, 5% from vehicle to drug conditions, no differences relative to vehicle were seen on the parameters of the psychometric function (asymptote, slope, or EC50) after drug administration. In contrast to findings in humans, our results suggest that modulation of 5HT activity has little impact on sucrose detectability at perithreshold concentrations in rats, at least at the doses used in this task. In the rat model, the purported paracrine/neurocrine action of serotonin in the taste bud may work in a manner that does not impact overt taste detection behavior.