Effects of interferon and encephalomyocarditis virus on in vitro development of preimplantation mouse embryos with and without the zona pellucida.
Effects of interferon and encephalomyocarditis virus on in vitro development of preimplantation mouse embryos with and without the zona pellucida.
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干扰素和脑心肌炎病毒对有透明带和无透明带的植入前小鼠胚胎体外发育的影响。
DOI：
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发表时间：
1984
期刊：
影响因子：
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通讯作者：
A. Ornoy
中科院分区：
文献类型：
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作者：
I. Zusman;D. Engelhard;P. Yaffe;A. Ron;A. Panet;A. Ornoy
采用体外培养法研究了植入前小鼠胚胎在干扰素（IFN）和小鼠脑心肌炎（EMC）病毒作用下，有无透明带的发育情况。胚胎（2- 8细胞期）从超排卵小鼠中获得，并在石蜡油下的改良Witten培养基中培养，在空气中37℃，5% CO2。去除透明带不影响胚胎的后续发育：90%的有透明带的胚胎和87%的没有透明带的胚胎达到了森胚-早期囊胚期。小鼠IFN（10(4)单位/ml）对有无透明带的着床前胚胎的发育能力无抑制作用：两组胚胎分别有88%和89%达到桑胚-早期囊胚期。植入前小鼠胚胎对EMC病毒的胚胎毒性作用非常敏感：每个胚胎感染20个感染颗粒时，43%的胚胎发育受到抑制。透明带没有显著的保护作用：去除透明带只略微改变着床前胚胎对病毒的易感性。用干扰素对胚胎进行预处理不能保护胚胎免受EMC病毒的胚胎毒性作用。这项工作表明，植入前小鼠胚胎似乎对IFN的抗病毒和抗增殖活性都有抵抗力。
Development of preimplantation mouse embryos, with or without the zona pellucida, in the presence of interferon (IFN) and mouse encephalomyocarditis (EMC) virus was studied using the in vitro culture method. The embryos (2- to 8-cell stages) were obtained from superovulated mice and cultured in modified Witten's medium under paraffin oil in 5% CO2 in air at 37 degrees C. Removal of the zona pellucida does not affect the subsequent development of the embryos: 90% of embryos with and 87% of embryos without the zona pellucida reached the morula-early blastocyst stages. Mouse IFN (10(4) units/ml) had no inhibitory effect on the developmental ability of the preimplantation embryos with or without the zona pellucida: 88 and 89% of the embryos in each group, respectively, reached the morula-early blastocyst stages. The preimplantation mouse embryos were sensitive to the embryotoxic effect of EMC virus: at a multiplicity of 20 infection particles per embryo the development of 43% of embryos was inhibited. The zona pellucida had no significant protective effect: Its removal changed only slightly the susceptibility of the preimplantation embryos to this virus. Pretreatment of embryos with IFN did not protect them from the embryotoxic effect of EMC virus. This work indicates that preimplantation mouse embryos appear to be resistant for both the antiviral and antiproliferative activities of IFN.