Occurrence of NDM-1, VIM-1, and OXA-10 Co-Producing Providencia rettgeri Clinical Isolate in China.
Occurrence of NDM-1, VIM-1, and OXA-10 Co-Producing Providencia rettgeri Clinical Isolate in China.
复制标题
NDM-1、VIM-1 和 OXA-10 联合生产雷氏普罗威登斯菌临床分离株在中国的出现情况
DOI：
10.3389/fcimb.2021.789646
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发表时间：
2021
影响因子：
5.7
通讯作者：
Hu F
中科院分区：
文献类型：
--
作者：
Shen S;Huang X;Shi Q;Guo Y;Yang Y;Yin D;Zhou X;Ding L;Han R;Yu H;Hu F
雷氏普罗威登斯菌是一种与医院获得性感染相关的尿路感染相关的医院病原体。近年来，已有报道雷氏毕赤酵母临床菌株产生新德里金属β-内酰胺酶（NDM）等降低抗菌药物治疗效果的β-内酰胺酶。然而，很少有报道在一个分离株中同时产生两种金属-β-内酰胺酶。在此，我们首次报道了共携带bla NDM-1、bla Vim-1和bla OXA-10的雷氏毕赤酵母菌株（P138）。微量肉汤稀释法药敏试验结果表明，雷氏毕赤酵母P138对美罗培南（MIC = 64μg/ml）、亚胺培南（MIC = 64 μ g/ml）和氨曲南（MIC = 32μg/ml）耐药。结合实验表明，携带blaNDM-1的质粒是可转移的。使用PCR检测碳青霉烯酶基因，并通过基于PCR的测序进行确认。使用Illumina鉴定了P. rettgeri的完整基因组序列，（Illumina，San Diego，CA，USA）短读测序（150 bp双端读段），并鉴定了许多常见的抗性基因，包括blaNDM-1，Vim-1，blaOXA-10，aac（6 '）-Il，aadA 5，ant（2“）-Ia，aadA 1，aac（6'）-Ib 3，aadA 1，aph（3 '）-Ia，aac（6 ′）-Ib-cr、qnrD 1、qnrA 1和catA 2。bla NDM-1基因的特征在于以下结构：IS 110-TnpA-IntI 1-aadB-IS 91-GroEL-GroES-DsbD-PAI-ble-bla NDM-1-IS 91-QnrS 1-IS 110。Blast比较显示bla NDM-1基因结构与质粒p5_SCL62具有>99%的相似性（99%核苷酸同一性和查询覆盖率）。总之，我们分离了共产生bla NDM-1、bla Vim-1和blaOXA-10的雷氏毕赤酵母菌株。据我们所知，这是世界上首次报道。需要密切监测菌株的发生。
Providencia rettgeri is a nosocomial pathogen associated with urinary tract infections related to hospital-acquired Infections. In recent years, P. rettgeri clinical strains producing New Delhi Metallo-β-lactamase (NDM) and other β-lactamase which reduce the efficiency of antimicrobial therapy have been reported. However, there are few reports of P. rettgeri co-producing two metallo-β-lactamases in one isolate. Here, we first reported a P. rettgeri strain (P138) co-harboring bla NDM-1, bla VIM-1, and bla OXA-10. The specie were identified using MALDI-TOF MS. The results of antimicrobial susceptibility testing by broth microdilution method indicated that P. rettgeri P138 was resistant to meropenem (MIC = 64μg/ml), imipenem (MIC = 64μg/ml), and aztreonam (MIC = 32μg/ml). Conjugation experiments revealed that the bla NDM-1-carrying plasmid was transferrable. The carbapenemase genes were detected using PCR and confirmed by PCR-based sequencing. The complete genomic sequence of the P. rettgeri was identified using Illumina (Illumina, San Diego, CA, USA) short-read sequencing (150bp paired-end reads), and many common resistance genes had been identified, including bla NDM-1, bla VIM-1, bla OXA-10, aac(6’)-Il, aadA5, ant(2’’)-Ia, aadA1, aac(6’)-Ib3, aadA1, aph(3’)-Ia, aac(6’)-Ib-cr, qnrD1, qnrA1, and catA2. The bla NDM-1 gene was characterized by the following structure: IS110–TnpA–IntI1–aadB–IS91–GroEL–GroES–DsbD–PAI–ble–bla NDM-1–IS91–QnrS1–IS110. Blast comparison revealed that the bla NDM-1 gene structure shared >99% similarity with plasmid p5_SCLZS62 (99% nucleotide identity and query coverage). In summary, we isolated a P. rettgeri strain coproducing bla NDM-1, bla VIM-1, and blaOXA-10. To the best of our acknowledge, this was first reported in the world. The occurrence of the strain needs to be closely monitored.