Distinct regulation of atonal in a visual organ of Drosophila: Organ-specific enhancer and lack of autoregulation in the larval eye.
Distinct regulation of atonal in a visual organ of Drosophila: Organ-specific enhancer and lack of autoregulation in the larval eye.
复制标题
果蝇视觉器官中无调性的独特调节：器官特异性增强子和幼虫眼中缺乏自动调节。
DOI：
10.1016/j.ydbio.2016.09.023
复制
发表时间：
2017
影响因子：
2.7
通讯作者：
Pignoni,Francesca
中科院分区：
文献类型：
--
作者：
Zhou,Qingxiang;Yu,Linlin;Friedrich,Markus;Pignoni,Francesca
Drosophilahas three types of visual organs, the larval eyes or Bolwig's organs (BO), the ocelli (OC) and the compound eyes (CE). In all, the bHLH protein Atonal (Ato) functions as the proneural factor for photoreceptors and effects the transition from progenitor cells to differentiating neurons. In this work, we investigate the regulation ofatoexpression in the BO primordium (BOP). Surprisingly, we find thatatotranscription in the BOP is entirely independent of the shared regulatory DNA for the developing CE and OC. The core enhancer for BOP expression, atoBO, lies ~6 kb upstream of theatogene, in contrast to the downstream location of CE and OC regulatory elements. Moreover, maintenance ofatoexpression in the neuronal precursors through autoregulation—a common and ancient feature ofatoexpression that is well-documented in eyes, ocelli and chordotonal organs—does not occur in the BO. We also show that the atoBOenhancer contains two binding sites for the transcription factor Sine oculis (So), a core component of the progenitor specification network in all three visual organs. These binding sites functionin vivoand are specifically bound by Soin vitro. Taken together, our findings reveal that the control ofatotranscription in the evolutionarily derived BO has diverged considerably fromatoregulation in the more ancestral compound eyes and ocelli, to the extent of acquiring what appears to be a distinct and evolutionarily novelcis-regulatory module.
