EXPRESSION OF AN ANIMAL VIRUS ANTIGENIC SITE ON THE SURFACE OF A PLANT-VIRUS PARTICLE
EXPRESSION OF AN ANIMAL VIRUS ANTIGENIC SITE ON THE SURFACE OF A PLANT-VIRUS PARTICLE
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DOI：
10.1006/viro.1993.1598
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发表时间：
1993-11-01
期刊：
影响因子：
3.7
通讯作者：
LOMONOSSOFF, GP
中科院分区：
文献类型：
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作者：
USHA, R;ROHLL, JB;LOMONOSSOFF, GP
为探讨豌豆花叶病毒颗粒是否可用于表达外源蛋白序列，将编码口蹄疫病毒Vp1表位的寡核苷酸克隆到豌豆花叶病毒基因组编码S外壳蛋白的区域。嵌合体的设计使得外源序列被表达为插入或替换部分野生型序列。虽然来自两个嵌合体的RNA都能够在豌豆原生质体中复制，但只有含有口蹄疫病毒序列作为插入的构建体能够直接形成衣壳并侵染整个豌豆植株。用该插入衍生物感染植物产生的修饰S蛋白可与口蹄疫病毒特异性抗血清反应。这些结果表明，CPMV可以用作抗原呈递系统，并增加了使用基于RNA病毒的载体在植物中生产疫苗的可能性。
To investigate if cowpea mosaic virus (CPMV) particles can be used to express foreign protein sequences, oligonucleotides encoding an epitope derived from VP1 of foot-and-mouth disease virus (FMDV) were cloned into the region of the CPMV genome encoding the small (S) coat protein. The chimeras were designed so that the foreign sequence was expressed either as an insertion or as a replacement for part of the wild-type sequence. While RNA from both chimeras was able to replicate in cowpea protoplasts only the construct containing the FMDV sequence as an insertion was able to direct capsid formation and infect whole cowpea plants. The modified S protein produced in plants infected with the insertion derivative reacted with FMDV-specific antiserum. These results show that CPMV can be used as an antigen presentation system and raises the possibility of producing vaccines in plants using a RNA virus-based vector.