Reduced nonspecific adsorption on covalently immobilized protein surfaces using poly(ethylene oxide) containing blocking agents
Reduced nonspecific adsorption on covalently immobilized protein surfaces using poly(ethylene oxide) containing blocking agents
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DOI：
10.1016/s0165-022x(03)00150-7
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发表时间：
2004-01-30
影响因子：
--
通讯作者：
Maes, G
中科院分区：
文献类型：
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作者：
Frederix, F;Bonroy, K;Maes, G
在许多应用中，例如DNA/蛋白质微阵列技术、酶联免疫吸附测定（ELISA）技术或表面等离子体共振（SPR）技术，都需要蛋白质与表面的共价偶联。在蛋白质的共价偶联之后，剩余的活性基团应该被阻断，以避免分析物与反应表面的共价结合。为此，最好使用含有避免非特异性吸附基团的阻断剂。这些阻断剂通常是乙醇胺和半胱氨酸，分别通过氨基和巯基进行蛋白质偶联。本报告提出了一种含有聚环氧乙烷（PEO）基团的新型阻断剂。这些阻断剂在避免非特异性吸附方面表现出增强的特性，因此在多功能蛋白质表面技术中具有优势。(C) 2003 Elsevier B.V.版权所有
In a number of applications, e.g. DNA/protein micro-array technology, enzyme-linked immunosorbent assay (ELISA) technology or surface plasmon resonance (SPR) technology, the covalent coupling of proteins to surfaces is required. Following the covalent coupling of proteins, the remaining reactive groups should be blocked in order to avoid covalent binding of the analyte to the reactive surface. To this end, preferably blocking agents containing groups that avoid nonspecific adsorption should be used. These blocking agents are typically ethanolamine and cysteine for protein coupling via amino groups and thiol groups, respectively. This report presents novel blocking agents containing poly(ethylene oxide) (PEO) groups. These blocking agents show enhanced qualities to avoid nonspecific adsorption and can therefore have advantages in versatile protein-surface technologies. (C) 2003 Elsevier B.V. All rights reserved.