TRANSPLANTABLE PROGENITORS OF NATURAL-KILLER-CELLS ARE DISTINCT FROM THOSE OF LYMPHOCYTE-T AND LYMPHOCYTE-B
TRANSPLANTABLE PROGENITORS OF NATURAL-KILLER-CELLS ARE DISTINCT FROM THOSE OF LYMPHOCYTE-T AND LYMPHOCYTE-B
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DOI：
10.1073/pnas.83.10.3427
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发表时间：
1986-05-01
影响因子：
11.1
通讯作者：
KUMAR, V
中科院分区：
文献类型：
--
作者：
HACKETT, J;BOSMA, GC;KUMAR, V
我们利用一种缺乏功能性T和B淋巴细胞的小鼠突变体（C.B-17 scid）来研究自然杀伤（NK）细胞、T细胞和B细胞的可移植祖细胞之间的关系。在将骨髓细胞转移到NK细胞耗尽和致死性照射的小鼠中后，通过其产生成熟NK细胞的能力检测骨髓中所含的NK祖细胞。通过两种不同的测定法监测受体小鼠中NK活性的再生：体内快速清除输注的YAC- 1细胞的能力和脾细胞体外裂解YAC-1细胞的能力。还检测了受体中是否存在促分裂原反应性T和B细胞以及前胸腺细胞（胸腺重建细胞）。我们发现C.B-17 scid骨髓细胞产生成熟NK细胞的能力与对照C.B-17骨髓细胞相当。再生的NK细胞具有相似的功能活性和表面表型。相反，来自C.B-17 scid小鼠的骨髓细胞不能产生胸腺细胞和外周T和B细胞。这些数据表明，可移植的NK祖细胞在C.B-17 scid小鼠中没有缺陷或缺乏，因此不同于T和B细胞的可移植祖细胞。
We have utilized a mouse mutant (C.B-17 scid) that lacks functional T and B lymphocytes to examine the relationships among transplantable progenitors of natural killer (NK) cells, T cells, and B cells. The NK-progenitor cells contained in the bone marrow were detected by their ability to generate mature NK cells, following transfer of bone marrow cells into NK cell-depleted and lethally irradiated mice. Regeneration of NK activity in the recipient mice was monitored by two different assays: the ability to rapidly clear infused YAC- 1 cells in vivo and the ability of spleen cells to lyse YAC-1 cells in vitro. Recipients were also tested for the presence of mitogen-responsive T and B cells and for prethymocytes (thymus-repopulating cells). We found that the capacity of C.B-17 scid bone marrow cells to generate mature NK cells was equivalent to that of control C.B-17 bone marrow cells. The regenerated NK cells shared similar functional activity and surface phenotype. In contrast, bone marrow cells from C.B-17 scid mice failed to generate thymocytes and peripheral T and B cells. These data indicate that the transplantable NK-progenitor cells are not defective or deficient in C.B-17 scid mice and, therefore, are distinct from the transplantable progenitor(s) of T and B cells.