Characterization of Mechanics and Cytocompatibility of Fibrin-Genipin Annulus Fibrosus Sealant with the Addition of Cell Adhesion Molecules
Characterization of Mechanics and Cytocompatibility of Fibrin-Genipin Annulus Fibrosus Sealant with the Addition of Cell Adhesion Molecules
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DOI：
10.1089/ten.tea.2012.0714
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发表时间：
2014-09-01
影响因子：
4.1
通讯作者：
Iatridis, James C.
中科院分区：
文献类型：
--
作者：
Guterl, Clare C.;Torre, Olivia M.;Iatridis, James C.
对于能够修复小的纤维环（AF）缺损的生物材料密封剂存在未满足的临床需求。这些缺陷的原因包括疼痛的椎间盘突出症，显微椎间盘切除术，椎间盘造影的针刺损伤相关的发病率，以及未来的髓核置换术。本研究描述了通过京尼平交联（FibGen）和添加细胞粘附分子（CAM）、纤连蛋白和胶原蛋白来增强纤维蛋白凝胶。使用一系列体外试验评估凝胶作为潜在AF密封剂的性能。含有CAM的FibGen凝胶与单独的纤维蛋白具有等同的粘附强度、基因表达、细胞形态学和细胞增殖。然而，与单独的纤维蛋白相比，FibGen凝胶具有增强的材料行为，其可调节至更高的剪切刚度值并接近人类纤维环组织，尺寸更稳定，并且具有更慢的体外降解速率。与单独的纤维蛋白相比，在FibGen凝胶上培养的人AF细胞的细胞形态学表现出增加的伸长率，并且向FibGen中添加CAM并不显著影响伸长率。这种FibGen凝胶有望用作修复小AF缺损的密封剂材料，或与其他生物材料结合用作较大缺损的粘合剂。
There is an unmet clinical need for a biomaterial sealant capable of repairing small annulus fibrosus (AF) defects. Causes of these defects include painful intervertebral disc herniations, microdiscectomy procedures, morbidity associated with needle puncture injury from discography, and future nucleus replacement procedures. This study describes the enhancements of a fibrin gel through genipin crosslinking (FibGen) and the addition of the cell adhesion molecules (CAMs), fibronectin and collagen. The gel's performance as a potential AF sealant is assessed using a series of in vitro tests. FibGen gels with CAMs had equivalent adhesive strength, gene expression, cytomorphology, and cell proliferation as fibrin alone. However, FibGen gels had enhanced material behaviors that were tunable to higher shear stiffness values and approximated human annulus tissue as compared with fibrin alone, were more dimensionally stable, and had a slower in vitro degradation rate. Cytomorphology of human AF cells cultured on FibGen gels exhibited increased elongation compared with fibrin alone, and the addition of CAMs to FibGen did not significantly affect elongation. This FibGen gel offers the promise of being used as a sealant material to repair small AF defects or to be used in combination with other biomaterials as an adhesive for larger defects.