HUMAN GLOMERULAR EPITHELIAL-CELL PROTEOGLYCANS
HUMAN GLOMERULAR EPITHELIAL-CELL PROTEOGLYCANS
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DOI：
10.1016/0003-9861(90)90224-m
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发表时间：
1990-04-01
影响因子：
3.9
通讯作者：
DAVIES, M
中科院分区：
文献类型：
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作者：
THOMAS, GJ;JENNER, L;DAVIES, M
由人肾小球上皮细胞培养物合成的蛋白聚糖已被分离和表征。检测到三种类型的硫酸乙酰肝素。硫酸乙酰肝素蛋白聚糖I（HSPG-I; Kav 6 B 0.04）被发现在细胞层和培养基中，占总蛋白聚糖合成的12%。HSPG-II（Kav6B0.25）占蛋白多糖的18%，位于培养基和细胞层。第三个群体（蛋白聚糖群体的9%），硫酸乙酰肝素糖胺聚糖（HS-GAG; Kav 6 B 0.4-0.8），具有与单个糖胺聚糖链或其片段一致的特性，仅在细胞层中发现。HSPG-I和HSPG-II从细胞层具有疏水性，它们被释放的细胞层温和的胰蛋白酶处理。HS-GAG缺乏这些特性，由低分子量硫酸乙酰肝素寡糖组成，并且在细胞内。释放到介质中的HSPG-I和-II缺乏疏水特性。这些细胞还产生三种不同类型的硫酸软骨素。在Sepharose CL-6 B柱的排除体积中洗脱的主要种类硫酸软骨素蛋白聚糖I（CSPG-I）占检测到的蛋白聚糖的30%，并且在细胞层和培养基中均发现。细胞层CSPG-I与辛基琼脂糖结合。通过温和的胰蛋白酶处理将其从细胞层释放。CSPG-II（Kav 6 B 0.1-0.23）占总35 S标记的大分子的10%，主要存在于培养基中。细胞提取物中存在少量CS-GAG（Kav 6 B 0.25-0.6），与HS-GAG一样，CS-GAG也存在于细胞内。脉冲追踪实验表明，HSPG-I和-II和CSPG-I和-II从细胞层中丢失，或者通过直接释放到培养基中，或者通过内化，在内化中它们被代谢成单个糖胺聚糖链，随后代谢成无机硫酸盐。
Proteoglycans synthesized by cultures of human glomerular epithelial cells have been isolated and characterized. Three types of heparan sulfate were detected. Heparan sulfate proteoglycan I (HSPG-I; Kav 6B 0.04) was found in the cell layer and medium and accounted for 12% of the total proteoglycans synthesized. HSPG-II (Kav 6B 0.25) accounted for 18% of the proteoglycans and was located in the medium and cell layer. A third population (9% of the proteoglycan population), heparan sulfate glycosaminoglycan (HS-GAG; Kav 6B 0.4-0.8), had properties consistent with single glycosaminoglycan chains or their fragments and was found only in the cell layer. HSPG-I and HSPG-II from the cell layer had hydrophobic properties; they were released from the cell layer by mild trypsin treatment. HS-GAG lacked these properties, consisted of low-molecular-mass heparan sulfate oligosaccharides, and were intracellular. HSPG-I and -II released to the medium lacked hydrophobic properties. The cells also produced three distinct types of chondroitin sulfates. The major species, chondroitin sulfate proteoglycan I (CSPG-I) eluted in the excluded volume of a Sepharose CL-6B column, accounted for 30% of the proteoglycans detected, and was found in both the cell layer and medium. Cell layer CSPG-I bound to octyl-Sepharose. It was released from the cell layer by mild trypsin treatment. CSPG-II (Kav 6B 0.1-0.23) accounted for 10% of the total 35S-labeled macromolecules and was found predominantly in the culture medium. A small amount of CS-GAG (Kav 6B 0.25-0.6) is present in the cell extract and like HS-GAG is intracellular. Pulse-chase experiments indicated that HSPG-I and -II and CSPG-I and -II are lost from the cell layer either by direct release into the medium or by internalization where they are metabolized to single glycosaminoglycan chains and subsequently to inorganic sulfate.