Cohesin is needed for bipolar mitosis in human cells
Cohesin is needed for bipolar mitosis in human cells
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DOI：
10.4161/cc.9.9.11525
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发表时间：
2010-05-01
期刊：
影响因子：
4.3
通讯作者：
Clarke, Duncan J.
中科院分区：
文献类型：
--
作者：
Diaz-Martinez, Laura A.;Beauchene, Nicole A.;Clarke, Duncan J.
多极有丝分裂与侵袭性癌症密切相关，是肿瘤级别的组织学诊断。然而，导致染色体分裂成两个以上纺锤极的因素还不清楚。本研究表明，人类细胞双极性有丝分裂需要内聚蛋白Rad21、Smc1和Smc3。在Rad21耗尽后，染色体在中期板上排列，双极纺锤体在大多数情况下组装，但在后期，分离的染色单体分离成多个极点。延时显微镜显示，在rad21缺失的中期细胞中，纺锤极经常发生分裂。有趣的是，外源表达不可切割的Rad21导致多极后期。由于黏结蛋白存在于有丝分裂的纺锤极，这些数据与黏结蛋白的非染色体功能一致。
Multi-polar mitosis is strongly linked with aggressive cancers and it is a histological diagnostic of tumor-grade. however, factors that cause chromosomes to segregate to more than two spindle poles are not well understood. here we show that cohesins Rad21, Smc1 and Smc3 are required for bipolar mitosis in human cells. After Rad21 depletion, chromosomes align at the metaphase plate and bipolar spindles assemble in most cases, but in anaphase the separated chromatids segregate to multiple poles. time-lapse microscopy revealed that the spindle poles often become split in Rad21-depleted metaphase cells. interestingly, exogenous expression of non-cleavable Rad21 results in multi-polar anaphase. Since cohesins are present at the spindle poles in mitosis, these data are consistent with a non-chromosomal function of cohesin.