Phylogenetic analyses of archaeal ribosomal DNA sequences from salt pan sediment of Mumbai, India
Phylogenetic analyses of archaeal ribosomal DNA sequences from salt pan sediment of Mumbai, India
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DOI：
10.1007/s00284-008-9167-z
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发表时间：
2008-08-01
影响因子：
2.6
通讯作者：
Qazi, Ghulam N.
中科院分区：
文献类型：
--
作者：
Ahmad, Nasier;Sharma, Sarika;Qazi, Ghulam N.
利用16 SrDNA分子生物学技术对印度孟买盐田沉积物中古菌多样性进行了研究。利用古细菌特异性引物，通过聚合酶链反应（PCR）扩增了盐田沉积物宏基因组的16 SrDNA片段。利用Hae 111和Msp 1两种内切酶对16 SrRNA基因进行PCR-RFLP分析，获得了32个独特的多态性类型。这两种类型的化石分布范围很广，包括泉古菌门和广古菌门。然而，没有检索到的泉古菌序列可以与以前培养的泉古菌。在所有的广古菌序列中，3个序列与盐古菌有关，2个序列与培养的甲烷八叠球菌有关，其余序列属于未培养的甲烷八叠球菌属，产甲烷菌属，和Methanolobus sp.测定的大部分序列与先前从各种海洋环境的宏基因组中获得的序列密切相关。首次调查的一个地点的系统发育研究揭示了一个高度多样化的古菌种群的存在，并可能代表了特别适应孟买盐田沉积物的新序列和生物。这些发现对于理解盐田生态系统的复杂性具有重要价值。
The archaeal diversity in salt pan sediment from Mumbai, India, was investigated by 16S rDNA-dependent molecular phylogeny. Small-subunit rRNA (16S rDNA) from salt pan sediment metagenome were amplified by polymerase chain reaction (PCR) using primers specific to the domain archaea. Thirty-two unique phylotypes were obtained by PCR-based RFLP of 16S rRNA genes using endonucleases Hae111 and Msp1, which were most suitable to score the genetic diversity. These phylotypes spanned a wide range within the domain Archaea including both Crenarchaeota and Euryarcheaota. However, none of the retrieved Crenarchaeota sequences could be grouped with previously cultured Crenarchaeota. Of all the Euryarcheaota sequences, three sequences were related to Haloarchaea, two were related to cultured Methanosarcina sp., and the remaining sequences were affiliated with uncultured Methanosarcina sp., Methanogenium sp., and Methanolobus sp. Most of the sequences determined were closely related to the sequences that had been previously obtained from metagenome of a variety of marine environments. The phylogenetic study of a site investigated for the first time revealed the presence of a highly diverse archaeal population and may represent novel sequences and organisms specially adapted to the salt pan sediment of Mumbai. These findings are of fundamental value for understanding the complexity of salt pan ecosystems.