Proteome analysis of proteins related to aggressive periodontitis combined with neutrophil chemotaxis dysfunction
Proteome analysis of proteins related to aggressive periodontitis combined with neutrophil chemotaxis dysfunction
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DOI：
10.1111/j.1600-051x.2010.01693.x
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发表时间：
2011-04-01
影响因子：
6.7
通讯作者：
Kurihara, Hidemi
中科院分区：
文献类型：
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作者：
Mizuno, Noriyoshi;Niitani, Miyuki;Kurihara, Hidemi
一些侵袭性牙周炎（agp）患者也表现出中性粒细胞趋化功能障碍。在这项研究中，我们试图通过蛋白质组学分析确定与中性粒细胞趋化功能障碍相关的Ag-P蛋白。材料与方法采用二维荧光差凝胶电泳系统检测4例Ag-P合并中性粒细胞趋化功能障碍患者与4例对照组中性粒细胞蛋白表达的差异。此外，采用实时聚合酶链反应（real-time polymerase chain reaction）检测上述方法鉴定的蛋白在四种类型的中性粒细胞中的mRNA水平：20例伴有或不伴有功能障碍的Ag-P患者，15例慢性牙周炎患者和15例对照组。结果Ag-P合并中性粒细胞功能障碍患者中性粒细胞中乳铁蛋白、caldesmon、热休克蛋白70和stac蛋白的表达水平高于对照组。Ag-P合并中性粒细胞功能障碍患者的中性粒细胞caldesmon mRNA水平高于其他两种牙周炎患者和对照组。结论caldesmon可能是Ag-P合并中性粒细胞趋化功能障碍的标志物。
P>AimSome patients suffering from aggressive periodontitis (Ag-P) also display neutrophil chemotaxis dysfunction. In this study, we attempted to identify the proteins involved in Ag-P associated with neutrophil chemotaxis dysfunction using proteome analysis.Material and MethodsA two-dimensional fluorescence difference gel electrophoresis system was used to detect differences in protein expression between neutrophils from four patients suffering from Ag-P combined with neutrophil chemotaxis dysfunction and those from four controls. Moreover, the mRNA levels of the proteins identified by the above method were examined in neutrophils from four types of subjects using the real-time polymerase chain reaction: twenty patients suffering from Ag-P with or without the dysfunction, 15 patients with chronic periodontitis, and 15 controls.ResultsFour proteins, lactoferrin, caldesmon, heat shock protein 70, and stac, displayed a higher protein expression level in the neutrophils from the patients suffering from Ag-P combined with the neutrophil dysfunction than in those from the control group. The caldesmon mRNA levels in the neutrophils from the patients suffering from Ag-P combined with the neutrophil dysfunction were high compared with those in the neutrophils from the patients suffering from the other two types of periodontitis and those from the control group.ConclusionCaldesmon may be a marker of Ag-P combined with neutrophil chemotaxis dysfunction.