Activity of human contact allergens in the murine local lymph node assay
Activity of human contact allergens in the murine local lymph node assay
复制标题
小鼠局部淋巴结测定中人类接触过敏原的活性
DOI：
10.1034/j.1600-0536.2000.043002095.x
复制
发表时间：
2000
影响因子：
5.5
通讯作者：
I. Kimber
中科院分区：
文献类型：
--
作者：
C. Ryan;G. Gerberick;L. Cruse;D. Basketter;L. Lea;L. Blaikie;R. Dearman;E. Warbrick;I. Kimber
小鼠局部淋巴结试验（LLNA）是一种预测性试验，用于鉴别可能导致皮肤致敏的化学物质。自最初开发以来，该检测方法一直是国家和国际评价研究的主题，并与豚鼠试验和人体数据进行了广泛比较。在这些研究的基础上，LLNA最近被ICCVAM（替代方法验证机构间协调委员会）认可为皮肤致敏危害识别的独立方法。与此同时，ICCVAM证实，尽管LLNA不是一种体外方法，但它确实代表了动物使用方式的改进，并可提供减少致敏危害评估中使用的动物数量的方法。本文所述的研究旨在进一步探索LLNA准确识别导致人类过敏性接触性皮炎的化学物质的能力。为此，我们在3个独立实验室中测量了总共18种测试化学品诱导的LLNA反应，其中11种已知会引起皮肤致敏，7种被认为与人类过敏性接触性皮炎的任何显著证据无关。LLNA正确分类了18种材料中的16种。与人类过敏性接触性皮炎相关的11种化学物质在LLNA中呈阳性。在7种被认为是非致敏剂的材料中，5种在LLNA中呈阴性，2种产生阳性结果。总的来说，这些数据提供了额外的证据，证明LLNA能够区分皮肤致敏剂和不具有显著皮肤致敏潜力的化学品，从而提供了一种危害识别方法，提供了重要的动物福利益处。
The murine local lymph node assay (LLNA) is a predictive test for the identification of chemicals that have the potential to cause skin sensitization. Since its original development, the assay has been the subject of national and international evaluation studies and extensive comparisons with guinea pig tests and human data. On the basis of these investigations, the LLNA has recently been endorsed by ICCVAM (Interagency Coordinating Committee on the Validation of Alternative Methods) as a stand‐alone method for skin sensitization hazard identification. At the same time, ICCVAM confirmed that, although the LLNA is not an in vitro method, it does represent a refinement in the way animals are used and can provide a means for reducing the number of animals used in sensitization hazard assessment. The investigations described here were designed to explore further the ability of the LLNA to identify accurately those chemicals that cause allergic contact dermatitis in humans. To that end we have measured, amongst 3 independent laboratories, LLNA responses induced by a total of 18 test chemicals, 11 of which are known to cause skin sensitization and 7 of which are believed not to be associated with any significant evidence of allergic contact dermatitis in humans. The LLNA correctly classified 16 of the 18 materials. The 11 chemicals tested which are associated with allergic contact dermatitis in humans were found to be positive in the LLNA. Of the 7 materials believed to be non‐sensitizers, 5 were negative in the LLNA and 2 produced positive results. Collectively, these data provide additional evidence that the LLNA is able to discriminate skin sensitizers from those chemicals which do not possess a significant skin sensitization potential and thus provides a method for hazard identification that offers important animal welfare benefits.