Os4BGlu14, a monolignol β‑Glucosidase, negatively affects seed longevity by influencing primary metabolism in rice
Os4BGlu14, a monolignol β‑Glucosidase, negatively affects seed longevity by influencing primary metabolism in rice
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DOI：
10.1007/s11103-020-01056-1
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
陈喜文
中科院分区：
文献类型：
--
作者：
任瑞娟;王培;王丽娜;苏京平;孙林静;孙玥;陈德富;陈喜文
Seed longevity is a crucial trait in agriculture and in the conservation of germplasm resources. β-Glucosidases.(BGlus) are multifunctional enzymes that affect plant growth and their adaptation to the environment. The function of rice.BGlus in seed longevity, however, remains unknown. We report here that Os4BGlu14, a rice β-Glucosidase, negatively.affected seed longevity during accelerated aging. Os4BGlu14 was highly expressed in rice embryos and induced by accelerated.aging. Compared to the wild type, rice lines overexpressing Os4BGlu14 had significantly greater grain length, but.smaller grain width and thickness. Overexpressing (OE) lines also showed lower starch but higher glucose contents. After.accelerated aging treatment, OE lines displayed a significantly lower germination percentage than the wild type. Additionally,.these lines had higher lignin accumulation before and after accelerated aging. Metabolome analysis detected 217.metabolites in untreated and aged rice seeds. Comparison of the differential metabolites between WT and OE5 revealed.that ten key metabolites, four of which (e.g., uridine 5′-diphosphoglucose-glucose, UDPG) were increased, while the other.six (e.g., γ-aminobutyric acid and methionine) were decreased, might be the crucial factors that lead to seed deterioration..Further analysis confirmed higher UDPG levels and more severe programmed cell death in OE lines than in the wild type..Furthermore, OE lines presented a lower germination rate after abscisic acid and paclobutrazol treatment during germination,.compared to the wild type. Our study provides a basis for understanding the function of Os4BGlu14 in seed longevity in rice.
